ArticlePloS one2015
High Concentrations of Atmospheric Ammonia Induce Alterations in the Hepatic Proteome of Broilers (Gallus gallus): An iTRAQ-Based Quantitative Proteomic Analysis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 43 citations in OpenAlex.
- Effects of Using Effective Microorganisms (EMs) and Zeolite on the Development of Productivity and Air and Litter Quality in Broiler Chicken Rearing.Animals : an open access journal from MDPI · 2026Article
- Effect of dietary interaction of sodium bentonite and litter treated with aluminum sulfate on growth performance, litter quality and serum biochemical indices of Nigerian Noiler birds.Tropical animal health and production · 2025Article
- Selenium Alleviates Ammonia-Induced Splenic Cell Apoptosis and Inflammation by Regulating the Interleukin Family/Death Receptor Axis and Nrf2 Signaling Pathway.Biological trace element research · 2023Article
- Influence of Improved Microclimate Conditions on Growth and Physiological Performance of Two Japanese Quail Lines.Animals : an open access journal from MDPI · 2023Article
- Geospatial Correlation Analysis between Air Pollution Indicators and Estimated Speed of COVID-19 Diffusion in the Lombardy Region (Italy).International journal of environmental research and public health · 2021Article
- Transcriptomic analysis of the liver in aged laying hens with different intensity of brown eggshell color.Animal bioscience · 2021Article
- Effects of ammonia on growth performance, lipid metabolism and cecal microbial community of rabbits.PloS one · 2021Article
- Skeletal Muscle and the Effects of Ammonia Toxicity in Fish, Mammalian, and Avian Species: A Comparative Review Based on Molecular Research.International journal of molecular sciences · 2020Review
- Effects of ammonia exposure on growth performance and cytokines in the serum, trachea, and ileum of broilers.Poultry science · 2020Article
- Genetic architecture related to contour feathers density in an F3 Biotech · 2019Article
- Ammonia production in poultry houses can affect health of humans, birds, and the environment-techniques for its reduction during poultry production.Environmental science and pollution research international · 2018Article
- Differential expression analysis of the broiler tracheal proteins responsible for the immune response and muscle contraction induced by high concentration of ammonia using iTRAQ-coupled 2D LC-MS/MS.Science China. Life sciences · 2016Article
- High concentrations of atmospheric ammonia induce alterations of gene expression in the breast muscle of broilers (Gallus gallus) based on RNA-Seq.BMC genomics · 2016Article
- Article
- Overgrazing induces alterations in the hepatic proteome of sheep (Proteome science · 2016Article
- Proteome changes in the small intestinal mucosa of growing pigs with dietary supplementation of non-starch polysaccharide enzymes.Proteome science · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the development of the poultry industry, ammonia, as a main contaminant in the air, is causing increasing problems with broiler health. To date, most studies of ammonia toxicity have focused on the nervous system and the gastrointestinal tract in mammals. However, few detailed studies have been conducted on the hepatic response to ammonia toxicity in poultry. The molecular mechanisms that underlie these effects remain unclear. In the present study, our group applied isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis to investigate changes in the protein profile change in hepatic tissue of broilers exposed to high concentrations of atmospheric ammonia, with the goal of characterizing the molecular mechanisms of chronic liver injury from exposure to high ambient levels of ammonia. Overall, 30 differentially expressed proteins that are involved in nutrient metabolism (energy, lipid, and amino acid), immune response, transcriptional and translational regulation, stress response, and detoxification were identified. In particular, two of these proteins, beta-1 galactosidase (GLB1) and a kinase (PRKA) anchor protein 8-like (AKAP8 L), were previously suggested to be potential biomarkers of chronic liver injury. In addition to the changes in the protein profile, serum parameters and histochemical analyses of hepatic tissue also showed extensive hepatic damage in ammonia-exposed broilers. Altogether, these findings suggest that longtime exposure to high concentrations of atmospheric ammonia can trigger chronic hepatic injury in broilers via different mechanisms, providing new information that can be used for intervention using nutritional strategies in the future.
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