Evidence map›Paper›PMID 25901990›Full record

ArticleThe Journal of clinical endocrinology and metabolism2015

Differential Response to High Glucose in Skin Fibroblasts of Monozygotic Twins Discordant for Type 1 Diabetes.

M Luiza Caramori, Youngki Kim, Rama Natarajan, Jason H Moore, Stephen S Rich, Josyf C Mychaleckyj, Ryoko Kuriyama, David Kirkpatrick, Michael Mauer

Open access · greenAbstract readTwin Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of clinical endocrinology and metabolism, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 3 countries.

M Luiza CaramoriDepartments of Medicine and Pediatrics (M.L.C., M.M.) and Pediatrics and Laboratory Medicine and Pathology (Y.K.), University of Minnesota, Minneapolis, Minnesota 55455; Department of Diabetes Complications, Obesity and Metabolism (R.N.), Beckman Research Institute, City of Hope, Duarte, California 91010; Department of Genetics (J.H.M.), Dartmouth College, Hanover, New Hampshire 03755; Departments of Public Health Sciences (S.S.R.) and Bioinformatics and Genetics (J.C.M.), University of Virginia, Charlottesville, Virginia 22908; and Department of Genetics, Cell Biology and Development (R.K., D.K.), University of Minnesota, Minneapolis, Minnesota 55455.
Youngki KimDepartments of Medicine and Pediatrics (M.L.C., M.M.) and Pediatrics and Laboratory Medicine and Pathology (Y.K.), University of Minnesota, Minneapolis, Minnesota 55455; Department of Diabetes Complications, Obesity and Metabolism (R.N.), Beckman Research Institute, City of Hope, Duarte, California 91010; Department of Genetics (J.H.M.), Dartmouth College, Hanover, New Hampshire 03755; Departments of Public Health Sciences (S.S.R.) and Bioinformatics and Genetics (J.C.M.), University of Virginia, Charlottesville, Virginia 22908; and Department of Genetics, Cell Biology and Development (R.K., D.K.), University of Minnesota, Minneapolis, Minnesota 55455.
Rama NatarajanDepartments of Medicine and Pediatrics (M.L.C., M.M.) and Pediatrics and Laboratory Medicine and Pathology (Y.K.), University of Minnesota, Minneapolis, Minnesota 55455; Department of Diabetes Complications, Obesity and Metabolism (R.N.), Beckman Research Institute, City of Hope, Duarte, California 91010; Department of Genetics (J.H.M.), Dartmouth College, Hanover, New Hampshire 03755; Departments of Public Health Sciences (S.S.R.) and Bioinformatics and Genetics (J.C.M.), University of Virginia, Charlottesville, Virginia 22908; and Department of Genetics, Cell Biology and Development (R.K., D.K.), University of Minnesota, Minneapolis, Minnesota 55455.
Jason H MooreDepartments of Medicine and Pediatrics (M.L.C., M.M.) and Pediatrics and Laboratory Medicine and Pathology (Y.K.), University of Minnesota, Minneapolis, Minnesota 55455; Department of Diabetes Complications, Obesity and Metabolism (R.N.), Beckman Research Institute, City of Hope, Duarte, California 91010; Department of Genetics (J.H.M.), Dartmouth College, Hanover, New Hampshire 03755; Departments of Public Health Sciences (S.S.R.) and Bioinformatics and Genetics (J.C.M.), University of Virginia, Charlottesville, Virginia 22908; and Department of Genetics, Cell Biology and Development (R.K., D.K.), University of Minnesota, Minneapolis, Minnesota 55455.
Stephen S RichDepartments of Medicine and Pediatrics (M.L.C., M.M.) and Pediatrics and Laboratory Medicine and Pathology (Y.K.), University of Minnesota, Minneapolis, Minnesota 55455; Department of Diabetes Complications, Obesity and Metabolism (R.N.), Beckman Research Institute, City of Hope, Duarte, California 91010; Department of Genetics (J.H.M.), Dartmouth College, Hanover, New Hampshire 03755; Departments of Public Health Sciences (S.S.R.) and Bioinformatics and Genetics (J.C.M.), University of Virginia, Charlottesville, Virginia 22908; and Department of Genetics, Cell Biology and Development (R.K., D.K.), University of Minnesota, Minneapolis, Minnesota 55455.
Josyf C MychaleckyjDepartments of Medicine and Pediatrics (M.L.C., M.M.) and Pediatrics and Laboratory Medicine and Pathology (Y.K.), University of Minnesota, Minneapolis, Minnesota 55455; Department of Diabetes Complications, Obesity and Metabolism (R.N.), Beckman Research Institute, City of Hope, Duarte, California 91010; Department of Genetics (J.H.M.), Dartmouth College, Hanover, New Hampshire 03755; Departments of Public Health Sciences (S.S.R.) and Bioinformatics and Genetics (J.C.M.), University of Virginia, Charlottesville, Virginia 22908; and Department of Genetics, Cell Biology and Development (R.K., D.K.), University of Minnesota, Minneapolis, Minnesota 55455.
Ryoko KuriyamaDepartments of Medicine and Pediatrics (M.L.C., M.M.) and Pediatrics and Laboratory Medicine and Pathology (Y.K.), University of Minnesota, Minneapolis, Minnesota 55455; Department of Diabetes Complications, Obesity and Metabolism (R.N.), Beckman Research Institute, City of Hope, Duarte, California 91010; Department of Genetics (J.H.M.), Dartmouth College, Hanover, New Hampshire 03755; Departments of Public Health Sciences (S.S.R.) and Bioinformatics and Genetics (J.C.M.), University of Virginia, Charlottesville, Virginia 22908; and Department of Genetics, Cell Biology and Development (R.K., D.K.), University of Minnesota, Minneapolis, Minnesota 55455.
David KirkpatrickDepartments of Medicine and Pediatrics (M.L.C., M.M.) and Pediatrics and Laboratory Medicine and Pathology (Y.K.), University of Minnesota, Minneapolis, Minnesota 55455; Department of Diabetes Complications, Obesity and Metabolism (R.N.), Beckman Research Institute, City of Hope, Duarte, California 91010; Department of Genetics (J.H.M.), Dartmouth College, Hanover, New Hampshire 03755; Departments of Public Health Sciences (S.S.R.) and Bioinformatics and Genetics (J.C.M.), University of Virginia, Charlottesville, Virginia 22908; and Department of Genetics, Cell Biology and Development (R.K., D.K.), University of Minnesota, Minneapolis, Minnesota 55455.
Michael MauerDepartments of Medicine and Pediatrics (M.L.C., M.M.) and Pediatrics and Laboratory Medicine and Pathology (Y.K.), University of Minnesota, Minneapolis, Minnesota 55455; Department of Diabetes Complications, Obesity and Metabolism (R.N.), Beckman Research Institute, City of Hope, Duarte, California 91010; Department of Genetics (J.H.M.), Dartmouth College, Hanover, New Hampshire 03755; Departments of Public Health Sciences (S.S.R.) and Bioinformatics and Genetics (J.C.M.), University of Virginia, Charlottesville, Virginia 22908; and Department of Genetics, Cell Biology and Development (R.K., D.K.), University of Minnesota, Minneapolis, Minnesota 55455.
Institut thématique Génétique, génomique et bioinformatique · FRPediatric Nephrology of Alabama · USUniversity of Virginia · USBeckman Research InstituteDiabetes Australia · AU

Funding

Inflammatory Gene Transcription In Diabetic ConditionsR01DK065073 · NIDDK · CITY OF HOPE/BECKMAN RESEARCH INSTITUTE · PI NATARAJAN, RAMA · 2003 to 2021
$7.9M
Epigenetic Markers of Complications and Metabolic Memory in the DCCT/EDIC cohort.DP3DK106917 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI NATARAJAN, RAMA · 2015 to 2015
$2.2M
NIDDK NIH HHS DP3 DK106917NIDDK NIH HHS R01 DK065073
6 · The paper itself

Abstract

contextMost epigenetic studies in diabetes compare normal cells in "high glucose" (HG) to cells in "normal glucose" (NG) and cells returned from HG to NG. Here we challenge this approach.

objectiveThe objective was to determine whether there were differences in gene expression in skin fibroblasts of monozygotic twins (MZT) discordant for type 1 diabetes (T1D).

designSkin fibroblasts were grown in NG (5.5 mmol/L) and HG (25 mmol/L) for multiple passages.

settingThis study was conducted at the University of Minnesota. PATIENTS: Patients were nine MZT pairs discordant for T1D. MAIN OUTCOME MEASURE(S): Gene expression was assessed by mRNA-Seq, using the Illumina HiSeq 2000 instrument. Pathway analysis tested directionally consistent group differences within the Kyoto Encyclopedia of Genes and Genomes pathways.

resultsA total of 3308 genes were differentially expressed between NG and HG in T1D MZT vs 889 in non-T1D twins. DNA replication, proteasome, cell cycle, base excision repair, homologous recombination, pyrimidine metabolism, and spliceosome pathways had overrepresented genes with increased expression in T1D twins with P values ranging from 7.21 × 10(-10) to 1.39 × 10(-4). In a companion article, we demonstrate that these pathway changes are related to diabetic nephropathy risk. There were no pathways statistically significant differently expressed in nondiabetic twins in HG vs NG.

conclusionsIn vivo exposure to diabetes alters cells in a manner that markedly changes their in vitro responses to HG. These results highlight the importance of using cells directly derived from diabetic patients for studies examining the effects of HG in diabetes.

Indexed as

AdultBlood GlucoseCells, CulturedDiabetes Mellitus, Type 1Dose-Response Relationship, DrugFemaleFibroblastsGene Expression ProfilingGene Expression RegulationHumansHyperglycemiaMaleMetabolic Networks and PathwaysMiddle AgedPrimary Cell CultureSkinBlood Glucose

Identifiers

PMID25901990
PMCPMC5393515
OpenAlexW2117358445

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.