ArticlePloS one2015
Combining microfluidics, optogenetics and calcium imaging to study neuronal communication in vitro.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Multicolored, Sonosensitizer-Optimized Organic Mechanoluminescent Nanoparticles for Functional Sono-Optogenetics.Journal of the American Chemical Society · 2026Article
- Harnessing Intelligence from Brain Cells In Vitro.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 2025Review
- Optogenetically enhanced physical reservoir computing withJournal of biomedical optics · 2025Article
- Closed-loop rehabilitation of upper-limb dyskinesia after stroke: from natural motion to neuronal microfluidics.Journal of neuroengineering and rehabilitation · 2025Review
- Article
- A programmable microfluidic platform to monitor calcium dynamics in microglia during inflammation.Microsystems & nanoengineering · 2024Article
- Network Bursts in 3D Neuron Clusters Cultured on Microcontact-Printed Substrates.Micromachines · 2023Article
- Portrait of intense communications within microfluidic neural networks.Scientific reports · 2023Article
- An Overview of In Vitro Biological Neural Networks for Robot Intelligence.Cyborg and bionic systems (Washington, D.C.) · 2023Review
- Microfluidics for Neuronal Cell and Circuit Engineering.Chemical reviews · 2022Review
- Brain physiome: A concept bridgingBioactive materials · 2022Review
- Retinitis Pigmentosa: Progress in Molecular Pathology and Biotherapeutical Strategies.International journal of molecular sciences · 2022Review
- Lab-on-Chip Microsystems forFrontiers in bioengineering and biotechnology · 2022Review
- The Future of Neurotoxicology: A Neuroelectrophysiological Viewpoint.Frontiers in toxicology · 2021Article
- A novel methodology to describe neuronal networks activity reveals spatiotemporal recruitment dynamics of synchronous bursting states.Journal of computational neuroscience · 2021Article
- Advances and prospects of rhodopsin-based optogenetics in plant research.Plant physiology · 2021Review
- Selective inhibition of gamma aminobutyric acid release from mouse hippocampal interneurone subtypes by the volatile anaesthetic isoflurane.British journal of anaesthesia · 2021Article
- Advances in 3D neuronal microphysiological systems: towards a functional nervous system on a chip.In vitro cellular & developmental biology. Animal · 2021Review
- Microdevice for directional axodendritic connectivity between micro 3D neuronal cultures.Microsystems & nanoengineering · 2021Article
- Is microfluidics the "assembly line" for CRISPR-Cas9 gene-editing?Biomicrofluidics · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper we report the combination of microfluidics, optogenetics and calcium imaging as a cheap and convenient platform to study synaptic communication between neuronal populations in vitro. We first show that Calcium Orange indicator is compatible in vitro with a commonly used Channelrhodopsine-2 (ChR2) variant, as standard calcium imaging conditions did not alter significantly the activity of transduced cultures of rodent primary neurons. A fast, robust and scalable process for micro-chip fabrication was developed in parallel to build micro-compartmented cultures. Coupling optical fibers to each micro-compartment allowed for the independent control of ChR2 activation in the different populations without crosstalk. By analyzing the post-stimuli activity across the different populations, we finally show how this platform can be used to evaluate quantitatively the effective connectivity between connected neuronal populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.