Evidence map›Paper›PMID 25901823›Full record

ArticleNature chemistry2015

Functionalization of cobalt porphyrin-phospholipid bilayers with his-tagged ligands and antigens.

Shuai Shao, Jumin Geng, Hyun Ah Yi, Shobhit Gogia, Sriram Neelamegham, Amy Jacobs, Jonathan F Lovell

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.

0numbers the graph read from it
0cells of the map it votes in
82citing papers in PubMed
6.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

82 citing papers in PubMed, 155 citations in OpenAlex.

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22 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Shuai Shao1] Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, USA [2] Department of Chemical and Biological Engineering, University at Buffalo, State University of New York. Buffalo, New York 14260, USA.
Jumin GengDepartment of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, USA.
Hyun Ah YiDepartment of Microbiology and Immunology, University at Buffalo, State University of New York, Buffalo, New York 14260, USA.
Shobhit GogiaDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York. Buffalo, New York 14260, USA.ORCID 0000000345749261
Sriram NeelameghamDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York. Buffalo, New York 14260, USA.
Amy JacobsDepartment of Microbiology and Immunology, University at Buffalo, State University of New York, Buffalo, New York 14260, USA.
Jonathan F Lovell1] Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260, USA [2] Department of Chemical and Biological Engineering, University at Buffalo, State University of New York. Buffalo, New York 14260, USA.
University at Buffalo, State University of New York · US

Funding

Light-Triggered Drug Release in Primed Pancreatic TumorsR01EB017270 · NIBIB · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI LOVELL, JONATHAN F · 2013 to 2021
$3.5M
VON WILLEBRAND FACTOR STRUCTURE-FUNCTION UNDE FLUID FLOWR01HL077258 · NHLBI · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI NEELAMEGHAM, SRIRAM · 2006 to 2015
$3.0M
Targeting Tumors with Resealable Nanovesicles Permeabilized by NIR LightDP5OD017898 · OD · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI LOVELL, JONATHAN F · 2013 to 2017
$1.9M
NHLBI NIH HHS HL77258NHLBI NIH HHS R01 HL077258NIBIB NIH HHS R01 EB017270NIBIB NIH HHS R01EB017270NIH HHS DP5 OD017898NIH HHS DP5OD017898
6 · The paper itself

Abstract

Methods to attach polypeptides to lipid bilayers are often indirect and ineffective, and can represent a substantial bottleneck in the formation of functionalized lipid-based materials. Although the polyhistidine tag (his-tag) has been transformative in its simplicity and efficacy in binding to immobilized metals, the successful application of this approach has been challenging in physiological settings. Here we show that lipid bilayers containing porphyrin-phospholipid conjugates that are chelated with cobalt, but not with other metals, can effectively capture his-tagged proteins and peptides. The binding follows a Co(II) to Co(III) transition and occurs within the sheltered hydrophobic bilayer, resulting in an essentially irreversible attachment in serum or in a million fold excess of competing imidazole. Using this approach we anchored homing peptides into the bilayer of preformed and cargo-loaded liposomes to enable tumour targeting without disrupting the bilayer integrity. As a further demonstration, a synthetic protein fragment derived from the human immunodeficiency virus was bound to immunogenic liposomes for potent antibody generation for an otherwise non-antigenic peptide.

Indexed as

CobaltLipid BilayersLiposomesPhospholipidsPorphyrinsCobaltLipid BilayersLiposomesPhospholipidsPorphyrins

Identifiers

PMID25901823
PMCPMC4408904
OpenAlexW2036832160

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.