ArticleJournal of the American Chemical Society2015
Uncovering the thermodynamics of monomer binding for RNA replication.
Article in Journal of the American Chemical Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Insight into the structures of unusual base pairs in RNA complexes containing a primer/template/adenosine ligand.RSC chemical biology · 2023Article
- Thermodynamics and kinetics of DNA and RNA dinucleotide hybridization to gaps and overhangs.Biophysical journal · 2023Article
- Direct monitoring of the thermodynamics and kinetics of DNA and RNA dinucleotide dehybridization from gaps and overhangs.bioRxiv : the preprint server for biology · 2023Article
- Sequencing the origins of life.BBA advances · 2022Review
- 2'/3' Regioselectivity of Enzyme-Free Copying of RNA Detected by NMR.Chembiochem : a European journal of chemical biology · 2020Article
- Probing complexity: thermodynamics and computational mechanics approaches to origins studies.Interface focus · 2019Article
- Structural Rationale for the Enhanced Catalysis of Nonenzymatic RNA Primer Extension by a Downstream Oligonucleotide.Journal of the American Chemical Society · 2018Article
- Synthesis of a Nonhydrolyzable Nucleotide Phosphoroimidazolide Analogue That Catalyzes Nonenzymatic RNA Primer Extension.Journal of the American Chemical Society · 2018Article
- Enzyme-free genetic copying of DNA and RNA sequences.Beilstein journal of organic chemistry · 2018Review
- A Kinetic Model of Nonenzymatic RNA Polymerization by Cytidine-5'-phosphoro-2-aminoimidazolide.Biochemistry · 2017Article
- Insight into the mechanism of nonenzymatic RNA primer extension from the structure of an RNA-GpppG complex.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Common and Potentially Prebiotic Origin for Precursors of Nucleotide Synthesis and Activation.Journal of the American Chemical Society · 2017Article
- Downstream Oligonucleotides Strongly Enhance the Affinity of GMP to RNA Primer-Template Complexes.Journal of the American Chemical Society · 2017Article
- Unusual Base-Pairing Interactions in Monomer-Template Complexes.ACS central science · 2016Article
- Oligoarginine peptides slow strand annealing and assist non-enzymatic RNA replication.Nature chemistry · 2016Article
- A Highly Reactive Imidazolium-Bridged Dinucleotide Intermediate in Nonenzymatic RNA Primer Extension.Journal of the American Chemical Society · 2016Article
- The effect of leaving groups on binding and reactivity in enzyme-free copying of DNA and RNA.Nucleic acids research · 2016Article
- Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The nonenzymatic replication of primordial RNA is thought to have been a critical step in the origin of life. However, despite decades of effort, the poor rate and fidelity of model template copying reactions have thus far prevented an experimental demonstration of nonenzymatic RNA replication. The overall rate and fidelity of template copying depend, in part, on the affinity of free ribonucleotides to the RNA primer-template complex. We have now used (1)H NMR spectroscopy to directly measure the thermodynamic association constants, Kas, of the standard ribonucleotide monophosphates (rNMPs) to native RNA primer-template complexes. The binding affinities of rNMPs to duplexes with a complementary single-nucleotide overhang follow the order C > G > A > U. Notably, these monomers bind more strongly to RNA primer-template complexes than to the analogous DNA complexes. The relative binding affinities of the rNMPs for complementary RNA primer-template complexes are in good quantitative agreement with the predictions of a nearest-neighbor analysis. With respect to G:U wobble base-pairing, we find that the binding of rGMP to a primer-template complex with a 5'-U overhang is approximately 10-fold weaker than to the complementary 5'-C overhang. We also find that the binding of rGMP is only about 2-fold weaker than the binding of rAMP to 5'-U, consistent with the poor fidelity observed in the nonenzymatic copying of U residues in RNA templates. The accurate Ka measurements for ribonucleotides obtained in this study will be useful for designing higher fidelity, more effective RNA replication systems.
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