ArticlePloS one2015
Resistance to Sri Lankan cassava mosaic virus (SLCMV) in genetically engineered cassava cv. KU50 through RNA silencing.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 64 citations in OpenAlex.
- Review
- Impact of Cassava Cultivars on Stylet Penetration Behavior and Settling ofPlants (Basel, Switzerland) · 2024Article
- Comparative transcriptomics analysis reveals defense mechanisms of Manihot esculenta Crantz against Sri Lanka Cassava MosaicVirus.BMC genomics · 2024Article
- Metabolic profiles of Sri Lankan cassava mosaic virus-infected and healthy cassava (Manihot esculenta Crantz) cultivars with tolerance and susceptibility phenotypes.BMC plant biology · 2023Article
- Article
- CRISPR/Cas9: an advanced platform for root and tuber crops improvement.Frontiers in genome editing · 2023Review
- Agrobacterium-mediated cassava transformation for the Asian elite variety KU50.Plant molecular biology · 2022Article
- RNA Interference: Promising Approach to Combat Plant Viruses.International journal of molecular sciences · 2022Review
- Development of a triple antibody sandwich enzyme-linked immunosorbent assay for cassava mosaic disease detection using a monoclonal antibody to Sri Lankan cassava mosaic virus.Virology journal · 2021Article
- Induction of resistance to sugarcane mosaic virus by RNA interference targeting coat protein gene silencing in transgenic sugarcane.Molecular biology reports · 2021Article
- RNAi technology for management of banana bunchy top disease.Food and energy security · 2020Review
- RNA silencing ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Engineering Disease-Resistant Cassava.Cold Spring Harbor perspectives in biology · 2019Review
- A rapid virus-induced gene silencing (VIGS) method for assessing resistance and susceptibility to cassava mosaic disease.Virology journal · 2017Article
- Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate.PloS one · 2017Article
- The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead.Frontiers in plant science · 2017Review
- Loss of CMD2-mediated resistance to cassava mosaic disease in plants regenerated through somatic embryogenesis.Molecular plant pathology · 2016Article
- Article
- The potential of using biotechnology to improve cassava: a review.In vitro cellular & developmental biology. Plant : journal of the Tissue Culture AssociationReview
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cassava ranks fifth among the starch producing crops of the world, its annual bioethanol yield is higher than for any other crop. Cassava cultivar KU50, the most widely grown cultivar for non-food purposes is susceptible to Sri Lankan cassava mosaic virus (SLCMV). The objective of this work was to engineer resistance to SLCMV by RNA interference (RNAi) in order to increase biomass yield, an important aspect for bioethanol production. Here, we produced transgenic KU50 lines expressing dsRNA homologous to the region between the AV2 and AV1 of DNA A of SLCMV. High level expression of dsRNA of SLCMV did not induce any growth abnormality in the transgenic plants. Transgenic lines displayed high levels of resistance to SLCMV compared to the wild-type plants and no virus load could be detected in uninoculated new leaves of the infected resistant lines after PCR amplification and RT-PCR analysis. The agronomic performance of the transgenic lines was unimpaired after inoculation with the virus as the plants presented similar growth when compared to the mock inoculated control plants and revealed no apparent reduction in the amount and weight of tubers produced. We show that the resistance is correlated with post-transcriptional gene silencing because of the production of transgene specific siRNA. The results demonstrate that transgenic lines exhibited high levels of resistance to SLCMV. This resistance coupled with the desirable yield components in the transgenic lines makes them better candidates for exploitation in the production of biomass as well as bioethanol.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.