ArticlePloS one2015
Integrated RNA-Seq and sRNA-Seq Analysis Identifies Chilling and Freezing Responsive Key Molecular Players and Pathways in Tea Plant (Camellia sinensis).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 113 citations in OpenAlex.
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- iTRAQ-Based Proteomic Analysis Reveals Molecular Pathways in Potato Cold Stress Response.Life (Basel, Switzerland) · 2025Article
- Exogenous strigolactones alleviate low-temperature stress in peppers seedlings by reducing the degree of photoinhibition.BMC plant biology · 2024Article
- Identification and expression analysis of the GLK gene family in tea plant (Camellia sinensis) and a functional study of CsGLK54 under low-temperature stress.Scientific reports · 2024Article
- Transcriptomic analysis ofFrontiers in plant science · 2024Article
- Comprehensive analysis of the laccase gene family in tea plant highlights its roles in development and stress responses.BMC plant biology · 2023Article
- Evolutionary Landscape of Tea Circular RNAs and Its Contribution to Chilling Tolerance of Tea Plant.International journal of molecular sciences · 2023Article
- Long-term cold, freezing and drought: overlapping and specific regulatory mechanisms and signal transduction in tea plant (Frontiers in plant science · 2023Article
- Integrative Omics Analysis of Three Oil Palm Varieties Reveals (Tanzania × Ekona) TE as a Cold-Resistant Variety in Response to Low-Temperature Stress.International journal of molecular sciences · 2022Article
- Genome-Wide Identification and Expression Analysis ofPlants (Basel, Switzerland) · 2022Article
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- Integrative analysis of microRNAs and mRNAs reveals the regulatory networks of triterpenoid saponin metabolism in Soapberry (Frontiers in plant science · 2022Article
- Integrated transcriptome and small RNA sequencing analyses reveal a drought stress response network in Sophora tonkinensis.BMC plant biology · 2021Article
- Transcriptome and Metabolome Analysis Revealed the Freezing Resistance Mechanism in 60-Year-Old OverwinteringBiology · 2021Article
- Transcriptome profiling of Malus sieversii under freezing stress after being cold-acclimated.BMC genomics · 2021Article
- MicroRNA Omics Analysis ofBiomolecules · 2021Article
- Integrated proteomic and metabolomic analyses reveal the importance of aroma precursor accumulation and storage in methyl jasmonate-primed tea leaves.Horticulture research · 2021Article
- Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tea [Camellia sinensis (L) O. Kuntze, Theaceae] is one of the most popular non-alcoholic beverages worldwide. Cold stress is one of the most severe abiotic stresses that limit tea plants' growth, survival and geographical distribution. However, the genetic regulatory network and signaling pathways involved in cold stress responses in tea plants remain unearthed. Using RNA-Seq, DGE and sRNA-Seq technologies, we performed an integrative analysis of miRNA and mRNA expression profiling and their regulatory network of tea plants under chilling (4℃) and freezing (-5℃) stress. Differentially expressed (DE) miRNA and mRNA profiles were obtained based on fold change analysis, miRNAs and target mRNAs were found to show both coherent and incoherent relationships in the regulatory network. Furthermore, we compared several key pathways (e.g., 'Photosynthesis'), GO terms (e.g., 'response to karrikin') and transcriptional factors (TFs, e.g., DREB1b/CBF1) which were identified as involved in the early chilling and/or freezing response of tea plants. Intriguingly, we found that karrikins, a new group of plant growth regulators, and β-primeverosidase (BPR), a key enzyme functionally relevant with the formation of tea aroma might play an important role in both early chilling and freezing response of tea plants. Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis further confirmed the results from RNA-Seq and sRNA-Seq analysis. This is the first study to simultaneously profile the expression patterns of both miRNAs and mRNAs on a genome-wide scale to elucidate the molecular mechanisms of early responses of tea plants to cold stress. In addition to gaining a deeper insight into the cold resistant characteristics of tea plants, we provide a good case study to analyse mRNA/miRNA expression and profiling of non-model plant species using next-generation sequencing technology.
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