ArticlePloS one2015
OpaR controls a network of downstream transcription factors in Vibrio parahaemolyticus BB22OP.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Integrons are anti-phage defence libraries in Vibrio parahaemolyticus.Nature microbiology · 2025Article
- Vibrio parahaemolyticus Down-Regulates the Intracellular c-di-GMP Level to Promote Swarming Motility by Sensing Surface.Current microbiology · 2024Article
- The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces.PLoS genetics · 2024Article
- Transcriptomic Profiles of Vibrio parahaemolyticus During Biofilm Formation.Current microbiology · 2023Article
- OpaR Exerts a Dynamic Control over c-di-GMP Homeostasis andMicrobiology spectrum · 2023Article
- A Class IV Adenylate Cyclase, CyaB, Is Required for Capsule Polysaccharide Production and Biofilm Formation in Vibrio parahaemolyticus.Applied and environmental microbiology · 2023Article
- Motility Control as a Possible Link Between Quorum Sensing to Surface Attachment in Vibrio Species.Advances in experimental medicine and biology · 2023Article
- Quorum sensing and QsvR tightly control the transcription ofFrontiers in microbiology · 2023Article
- QsvR and OpaR coordinately repress biofilm formation byFrontiers in microbiology · 2023Article
- Computationally Designed Anti-LuxP DNA Aptamer Suppressed Flagellar Assembly- and Quorum Sensing-Related Gene Expression inBiology · 2022Article
- Roles of the second messenger c-di-GMP in bacteria: Focusing on the topics of flagellar regulation and Vibrio spp.Genes to cells : devoted to molecular & cellular mechanisms · 2022Review
- Regulatory Small RNA Qrr2 Is Expressed Independently of Sigma Factor-54 and Can Function as the Sole Qrr Small RNA To Control Quorum Sensing in Vibrio parahaemolyticus.Journal of bacteriology · 2022Article
- Comparative Transcriptome Analysis Reveals Regulatory Factors Involved inFrontiers in cellular and infection microbiology · 2022Article
- Amino acid divergence in the ligand-binding pocket of Vibrio LuxR/HapR proteins determines the efficacy of thiophenesulfonamide inhibitors.Molecular microbiology · 2021Article
- Quorum sensing inPeerJ · 2021Article
- OpaR Controls the Metabolism of c-di-GMP inFrontiers in microbiology · 2021Article
- Fis Connects Two Sensory Pathways, Quorum Sensing and Surface Sensing, to Control Motility inFrontiers in microbiology · 2021Article
- Article
- Quorum-Sensing Regulator OpaR Directly Represses Seven Protease Genes inFrontiers in microbiology · 2020Article
- Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology.Journal of microbiology (Seoul, Korea) · 2019Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Vibrio parahaemolyticus is an emerging world-wide human pathogen that is associated with food-borne gastroenteritis when raw or undercooked seafood is consumed. Expression of virulence factors in this organism is modulated by the phenomenon known as quorum sensing, which permits differential gene regulation at low versus high cell density. The master regulator of quorum sensing in V. parahaemolyticus is OpaR. OpaR not only controls virulence factor gene expression, but also the colony and cellular morphology associated with growth on a surface and biofilm formation. Whole transcriptome Next Generation sequencing (RNA-Seq) was utilized to determine the OpaR regulon by comparing strains BB22OP (opaR+, LM5312) and BB22TR (∆opaR1, LM5674). This work, using the published V. parahaemolyticus BB22OP genome sequence, confirms and expands upon a previous microarray analysis for these two strains that used an Affymetrix GeneChip designed from the closely related V. parahaemolyticus RIMD2210633 genome sequence. Overall there was excellent correlation between the microarray and RNA-Seq data. Eleven transcription factors under OpaR control were identified by both methods and further confirmed by quantitative reverse transcription PCR (qRT-PCR) analysis. Nine of these transcription factors were demonstrated to be direct OpaR targets via in vitro electrophoretic mobility shift assays with purified hexahistidine-tagged OpaR. Identification of the direct and indirect targets of OpaR, including small RNAs, will enable the construction of a network map of regulatory interactions important for the switch between the nonpathogenic and pathogenic states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.