ArticlePLoS pathogens2015
GATA-Dependent Glutaminolysis Drives Appressorium Formation in Magnaporthe oryzae by Suppressing TOR Inhibition of cAMP/PKA Signaling.
Article in PLoS pathogens, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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68 citing papers in PubMed, 118 citations in OpenAlex.
- Review
- Functional analysis of target of rapamycin in regulating mycelial growth and development in Penicillium italicum.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Green synthesis of silver nanoparticles using fermentation extracts from a mangrove soil bacterium: morphological characterization, and antifungal activities against rice blast fungus.Pest management science · 2026Article
- Regulator of G-Protein Signalling Protein AaRgs2 Negatively Regulates Appressorium-Like Formation of Alternaria alternata Induced by Pear Cutin Monomer via the AaRgs2-AaGα1-AaAC Module.Molecular plant pathology · 2026Article
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- The SsSmk1-SsSom1-SsMsb2 Pathway Regulates Infection Cushion Formation and Pathogenicity in Sclerotinia sclerotiorum.Molecular plant pathology · 2025Article
- Mechanisms of regulated cell death during plant infection by the rice blast fungus Magnaporthe oryzae.Cell death and differentiation · 2025Review
- Live cell imaging of plant infection provides new insight into the biology of pathogenesis by the rice blast fungus Magnaporthe oryzae.Journal of microscopy · 2025Review
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- Harnessing the Tet-Off system for effective gene silencing inFrontiers in plant science · 2025Article
- The TOR signalling pathway in fungal phytopathogens: A target for plant disease control.Molecular plant pathology · 2024Review
- Functional Characterization of the Transcription Factor GeneJournal of fungi (Basel, Switzerland) · 2024Article
- Pyricularia oryzae: Lab star and field scourge.Molecular plant pathology · 2024Review
- Molecular basis for loss of virulence inFrontiers in plant science · 2024Article
- Regulation of nutrient utilization in filamentous fungi.Applied microbiology and biotechnology · 2023Review
- A New Insight into 6-Pentyl-2H-pyran-2-one againstJournal of fungi (Basel, Switzerland) · 2023Article
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- Constitutive activation of TORC1 signalling attenuates virulence in the cross-kingdom fungal pathogen Fusarium oxysporum.Molecular plant pathology · 2023Article
- Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal plant pathogens are persistent and global food security threats. To invade their hosts they often form highly specialized infection structures, known as appressoria. The cAMP/ PKA- and MAP kinase-signaling cascades have been functionally delineated as positive-acting pathways required for appressorium development. Negative-acting regulatory pathways that block appressorial development are not known. Here, we present the first detailed evidence that the conserved Target of Rapamycin (TOR) signaling pathway is a powerful inhibitor of appressorium formation by the rice blast fungus Magnaporthe oryzae. We determined TOR signaling was activated in an M. oryzae mutant strain lacking a functional copy of the GATA transcription factor-encoding gene ASD4. Δasd4 mutant strains could not form appressoria and expressed GLN1, a glutamine synthetase-encoding orthologue silenced in wild type. Inappropriate expression of GLN1 increased the intracellular steady-state levels of glutamine in Δasd4 mutant strains during axenic growth when compared to wild type. Deleting GLN1 lowered glutamine levels and promoted appressorium formation by Δasd4 strains. Furthermore, glutamine is an agonist of TOR. Treating Δasd4 mutant strains with the specific TOR kinase inhibitor rapamycin restored appressorium development. Rapamycin was also shown to induce appressorium formation by wild type and Δcpka mutant strains on non-inductive hydrophilic surfaces but had no effect on the MAP kinase mutant Δpmk1. When taken together, we implicate Asd4 in regulating intracellular glutamine levels in order to modulate TOR inhibition of appressorium formation downstream of cPKA. This study thus provides novel insight into the metabolic mechanisms that underpin the highly regulated process of appressorium development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.