ArticleClinical epigenetics2015
DNA methylation differences between in vitro- and in vivo-conceived children are associated with ART procedures rather than infertility.
Article in Clinical epigenetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 1 synthesis or guideline pooled it, 113 citations in OpenAlex.
- Association of medically assisted reproduction with offspring cord blood DNA methylation across cohorts.Human reproduction (Oxford, England) · 2021Pooled it
- Epigenetic safety of in vitro maturation in PCOS: genome-wide DNA methylation profiling of cord blood from a randomized controlled trial.Journal of ovarian research · 2026Trial
- Article
- Modest increase in the de novo single-nucleotide mutation rate in house mice born by assisted reproduction.Genome research · 2026Article
- Crosstalk between H3K4me3 and oxidative stress is a potential target for the improvement of ART-derived embryos.Nature communications · 2025Article
- Differential Effects of Assisted Reproduction Technology on Placental Epigenetics and Angiogenesis: Insights from Fresh, Frozen, and Egg Donation Pregnancies.Life (Basel, Switzerland) · 2025Article
- Supplementation with oviductal EVs from the estrus, metestrus, and diestrus stages improved developmental competence of IVF mouse embryos.Scientific reports · 2025Article
- Increased rate ofbioRxiv : the preprint server for biology · 2025Article
- Oviductal extracellular matrix hydrogels enhance in vitro culture of rabbit embryos and reduce deficiencies during assisted reproductive technologies.Scientific reports · 2024Article
- Decoding the Epigenetics of Infertility: Mechanisms, Environmental Influences, and Therapeutic Strategies.Epigenomes · 2024Review
- Long-term health risk of offspring born from assisted reproductive technologies.Journal of assisted reproduction and genetics · 2024Review
- Frequency, morbidity and equity - the case for increased research on male fertility.Nature reviews. Urology · 2024Review
- Pregnancy by Oocyte Donation: Reviewing Fetal-Maternal Risks and Complications.International journal of molecular sciences · 2023Review
- Pathomechanisms of Prenatally Programmed Adult Diseases.Antioxidants (Basel, Switzerland) · 2023Review
- Hormonal stimulation reduces numbers and impairs function of human uterine natural killer cells during implantation.Human reproduction (Oxford, England) · 2023Article
- The X-factor in ART: does the use of assisted reproductive technologies influence DNA methylation on the X chromosome?Human genomics · 2023Article
- Embryo cryopreservation leads to sex-specific DNA methylation perturbations in both human and mouse placentas.Human molecular genetics · 2022Article
- Clomifene and Assisted Reproductive Technology in Humans Are Associated with Sex-Specific Offspring Epigenetic Alterations in Imprinted Control Regions.International journal of molecular sciences · 2022Article
- DNA Methylation in Offspring Conceived after Assisted Reproductive Techniques: A Systematic Review and Meta-Analysis.Journal of clinical medicine · 2022Review
- Biochemical Hazards during Three Phases of Assisted Reproductive Technology: Repercussions Associated with Epigenesis and Imprinting.International journal of molecular sciences · 2022Review
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8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundWe, and others, have demonstrated previously that there are differences in DNA methylation and transcript levels of a number of genes in cord blood and placenta between children conceived using assisted reproductive technologies (ART) and children conceived in vivo. The source of these differences (the effect of ART versus the underlying infertility) has never been determined in humans. In this study, we have attempted to resolve this issue by comparing placental DNA methylation levels at 37 CpG sites in 16 previously identified candidate genes in independent populations of children conceived in vivo ('fertile control' group) with ART children conceived from two groups: either autologous oocytes with infertility in one or both parents ('infertile ART' group) or donor oocytes (obtained from young fertile donors) without male infertility ('donor oocyte ART' group).
resultsOf the 37 CpG sites analyzed, significant differences between the three groups were found in 11 CpGs (29.73 %), using ANOVA. Tukey's post hoc test on the significant results indicated that seven (63.63 %) of these differences were significant between the donor oocyte ART and fertile control groups. In addition, 20 of the 37 CpGs analyzed had been identified as differentially methylated between ART and fertile control groups in an independent population in a prior study. Of these 20 CpG sites, 9 also showed significant differences in the present population. An additional 9 CpGs were found to be significantly different between the two groups. Of these 18 candidate CpGs, 12 CpGs (in seven candidate genes) also showed significant differences in placental DNA methylation levels between the donor oocyte ART and fertile control groups.
conclusionsThese data suggest strongly that the DNA methylation differences observed between ART and in vivo conceptions are associated with some aspect of ART protocols, not simply the underlying infertility.
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