ArticlePlant methods2015
Image analysis of anatomical traits in stalk transections of maize and other grasses.
Article in Plant methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- The Zma-miRNA319-ZmMYB74 Module Regulates Maize Resistance to Stalk Rot Disease by Modulating Lignin Deposition.Plant biotechnology journal · 2026Article
- Association analysis of maize stem vascular bundle micro-characteristics with yield components based on micro-CT and identification of related genes.Scientific reports · 2025Article
- A robust and efficient automatic method to segment maize FASGA stained stem cross section images to accurately quantify histological profile.Plant methods · 2022Article
- High-Throughput Phenotyping Accelerates the Dissection of the Phenotypic Variation and Genetic Architecture of Shank Vascular Bundles in Maize (Plants (Basel, Switzerland) · 2022Article
- Cell Wall Composition Impacts Structural Characteristics of the Stems and Thereby the Biomass Yield.Journal of agricultural and food chemistry · 2022Article
- Transcriptome analysis reveals the mechanism of internode development affecting maize stalk strength.BMC plant biology · 2022Article
- High throughput phenotyping of cross-sectional morphology to assess stalk lodging resistance.Plant methods · 2022Article
- Responses of Maize Internode to Water Deficit Are Different at the Biochemical and Histological Levels.Frontiers in plant science · 2021Article
- Darkfield and Fluorescence Macrovision of a Series of Large Images to Assess Anatomical and Chemical Tissue Variability in Whole Cross-Sections of Maize Stems.Frontiers in plant science · 2021Article
- Dissecting the phenotypic components and genetic architecture of maize stem vascular bundles using high-throughput phenotypic analysis.Plant biotechnology journal · 2021Article
- Phenotyping analysis of maize stem using micro-computed tomography at the elongation and tasseling stages.Plant methods · 2020Article
- A novel rind puncture technique to measure rind thickness and diameter in plant stalks.Plant methods · 2020Article
- Machine Learning Approaches to Improve Three Basic Plant Phenotyping Tasks Using Three-Dimensional Point Clouds.Plant physiology · 2019Article
- Genome-wide association analysis of stalk biomass and anatomical traits in maize.BMC plant biology · 2019Article
- Crop Phenomics: Current Status and Perspectives.Frontiers in plant science · 2019Review
- High-throughput micro-phenotyping measurements applied to assess stalk lodging in maize (Zea mays L.).Biological research · 2018Article
- Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography.Journal of visualized experiments : JoVE · 2018Article
- PlantSize Offers an Affordable, Non-destructive Method to Measure Plant Size and ColorFrontiers in plant science · 2018Article
- The elastic modulus for maize stems.Plant methods · 2018Article
- A Statistical Description of Plant Shoot Architecture.Current biology : CB · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGrass stalks architecturally support leaves and reproductive structures, functionally support the transport of water and nutrients, and are harvested for multiple agricultural uses. Research on these basic and applied aspects of grass stalks would benefit from improved capabilities for measuring internal anatomical features. In particular, methods suitable for phenotyping populations of plants are needed.
resultsTo meet the need for large-scale measurements of stalk anatomy features, we developed custom image processing software that utilized a variety of global thresholding, local filtering, and feature detection methods to measure rind thickness, pith area, vascular bundle counts, and individual vascular bundle size from digital images of hand-cut transections of stalks collected with a flatbed document scanner. The tool determined vascular bundle number with an average accuracy of 90% across maize genotypes that varied five-fold for this trait. The method is demonstrated on maize, sorghum, and Miscanthus stalks. The computer source code is staged for download.
conclusionsSimplicity of sample preparation and semi-automated analyses enabled by this tool greatly increase measurement throughput relative to standard microscopy-based techniques while maintaining high accuracy. The tool is expected to be useful in genetic and physiological studies of the relationships between stalk anatomy and traits such as biofuel suitability, water use efficiency, or nutrient transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.