ArticleInternational journal for parasitology2015
Biogenesis of the crystalloid organelle in Plasmodium involves microtubule-dependent vesicle transport and assembly.
Article in International journal for parasitology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 21 citations in OpenAlex.
- A Genetically Encoded Marker for Imaging of Internal Plasmodium Membranes.Molecular microbiology · 2026Article
- Targeting protein-protein interactions in Plasmodium: from asexual replication to sexual development.Parasites & vectors · 2026Review
- A pleckstrin homology domain protein is involved in crystalloid formation inFrontiers in cellular and infection microbiology · 2026Article
- Decoding the role of microtubules: a trafficking road for vesicle.Theranostics · 2025Review
- Article
- The molecular mechanisms driving Plasmodium cell division.Biochemical Society transactions · 2024Review
- The Multiple Roles of LCCL Domain-Containing Proteins for Malaria Parasite Transmission.Microorganisms · 2024Review
- Identification of genes required for Plasmodium gametocyte-to-sporozoite development in the mosquito vector.Cell host & microbe · 2023Article
- Identification of a novel AP2 transcription factor in zygotes with an essential role in Plasmodium ookinete development.PLoS pathogens · 2022Article
- Identification of CCp5 and FNPA as Novel Non-canonical Members of the CCp Protein Family inFrontiers in veterinary science · 2022Article
- Plasmodium berghei LAPs form an extended protein complex that facilitates crystalloid targeting and biogenesis.Journal of proteomics · 2020Article
- NAD(P) transhydrogenase has vital non-mitochondrial functions in malaria parasite transmission.EMBO reports · 2020Article
- Dysregulated gene expression in oocysts of Plasmodium berghei LAP mutants.Molecular and biochemical parasitology · 2019Article
- Identification of a PH domain-containing protein which is localized to crystalloid bodies of Plasmodium ookinetes.Malaria journal · 2018Article
- The Plasmodium LAP complex affects crystalloid biogenesis and oocyst cell division.International journal for parasitology · 2018Article
- LCCL protein complex formation in Plasmodium is critically dependent on LAP1.Molecular and biochemical parasitology · 2017Article
- Article
- Dynamic protein S-palmitoylation mediates parasite life cycle progression and diverse mechanisms of virulence.Critical reviews in biochemistry and molecular biology · 2017Review
- Maternally supplied S-acyl-transferase is required for crystalloid organelle formation and transmission of the malaria parasite.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Malaria parasites possess unique subcellular structures and organelles. One of these is the crystalloid, a multivesicular organelle that forms during the parasite's development in vector mosquitoes. The formation and function of these organelles remain poorly understood. A family of six conserved and modular proteins named LCCL-lectin adhesive-like proteins (LAPs), which have essential roles in sporozoite transmission, localise to the crystalloids. In this study we analyse crystalloid formation using transgenic Plasmodium berghei parasites expressing GFP-tagged LAP3. We show that deletion of the LCCL domain from LAP3 causes retarded crystalloid development, while knockout of LAP3 prevents formation of the organelle. Our data reveal that the process of crystalloid formation involves active relocation of endoplasmic reticulum-derived vesicles to common assembly points via microtubule-dependent transport. Inhibition of microtubule-dependent cargo transport disrupts this process and replicates the LCCL domain deletion mutant phenotype in wildtype parasites. These findings provide the first clear insight into crystalloid biogenesis, demonstrating a fundamental role for the LAP family in this process, and identifying the crystalloid and its formation as potential targets for malaria transmission control.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.