ArticleMucosal immunology2016
High-dimensional immune profiling of total and rotavirus VP6-specific intestinal and circulating B cells by mass cytometry.
Article in Mucosal immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Analysis of Plasmablasts From Children With Kawasaki Disease Reveals Evidence of a Convergent Antibody Response to a Specific Protein Epitope.The Journal of infectious diseases · 2023Article
- Establishment of isotype-switched, antigen-specific B cells in multiple mucosal tissues using non-mucosal immunization.NPJ vaccines · 2023Article
- Immunity to enteric viruses.Immunity · 2022Review
- Single-cell approaches to dissect adaptive immune responses involved in autoimmunity: the case of celiac disease.Mucosal immunology · 2022Review
- Development and function of tissue-resident memory B cells.Advances in immunology · 2022Article
- Surface phenotypes of naive and memory B cells in mouse and human tissues.Nature immunology · 2022Article
- B and T Cell Immunity in Tissues and Across the Ages.Vaccines · 2021Review
- Article
- Origins and clonal convergence of gastrointestinal IgEScience immunology · 2020Article
- Programming Isotype-Specific Plasma Cell Function.Trends in immunology · 2019Review
- Prostate-specific markers to identify rare prostate cancer cells in liquid biopsies.Nature reviews. Urology · 2019Review
- Proof of concept study of mass cytometry in septic shock patients reveals novel immune alterations.Scientific reports · 2018Article
- Advancing systems immunology through data-driven statistical analysis.Current opinion in biotechnology · 2018Review
- Rotavirus vaccines: why continued investment in research is necessary.Current clinical microbiology reports · 2018Article
- Gut Mucosal Antibody Responses and Implications for Food Allergy.Frontiers in immunology · 2018Review
- Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals.Immunity · 2017Article
- VP4- and VP7-specific antibodies mediate heterotypic immunity to rotavirus in humans.Science translational medicine · 2017Article
- Antibody-secreting plasma cells persist for decades in human intestine.The Journal of experimental medicine · 2017Article
- The human intestinal B-cell response.Mucosal immunology · 2016Review
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
In-depth phenotyping of human intestinal antibody secreting cells (ASCs) and their precursors is important for developing improved mucosal vaccines. We used single-cell mass cytometry to simultaneously analyze 34 differentiation and trafficking markers on intestinal and circulating B cells. In addition, we labeled rotavirus (RV) double-layered particles with a metal isotope and characterized B cells specific to the RV VP6 major structural protein. We describe the heterogeneity of the intestinal B-cell compartment, dominated by ASCs with some phenotypic and transcriptional characteristics of long-lived plasma cells. Using principal component analysis, we visualized the phenotypic relationships between major B-cell subsets in the intestine and blood, and revealed that IgM(+) memory B cells (MBCs) and naive B cells were phenotypically related as were CD27(-) MBCs and switched MBCs. ASCs in the intestine and blood were highly clonally related, but associated with distinct trajectories of phenotypic development. VP6-specific B cells were present among diverse B-cell subsets in immune donors, including naive B cells, with phenotypes representative of the overall B-cell pool. These data provide a high dimensional view of intestinal B cells and the determinants regulating humoral memory to a ubiquitous, mucosal pathogen at steady-state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.