ArticlePlant biotechnology journal2016
Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- Inspired by taxol: biosynthesis and engineering of hypericin and other phytochemicals in Hypericum.Planta · 2026Review
- Recent advances inFrontiers in plant science · 2026Review
- A new 13-lipoxygenase from Taxus media callus is functionally linked to paclitaxel biosynthesis.Scientific reports · 2025Article
- Article
- Transcriptome and Metabolome Analyses ofPlants (Basel, Switzerland) · 2025Article
- Comprehensive strategies for paclitaxel production: insights from plant cell culture, endophytic microorganisms, and synthetic biology.Horticulture research · 2025Article
- Recent advances in paclitaxel biosynthesis and regulation.Journal of experimental botany · 2025Review
- Post-genomic illumination of paclitaxel biosynthesis.Nature plants · 2024Review
- Biosynthesis of the highly oxygenated tetracyclic core skeleton of Taxol.Nature communications · 2024Article
- Characterization of lipid droplets from a Taxus media cell suspension and their potential involvement in trafficking and secretion of paclitaxel.Plant cell reports · 2022Article
- Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated byMolecules (Basel, Switzerland) · 2021Review
- Recent Advances in Metabolic Engineering, Protein Engineering, and Transcriptome-Guided Insights Toward Synthetic Production of Taxol.Frontiers in bioengineering and biotechnology · 2021Review
- Biochemical characterization of acyl activating enzymes for side chain moieties of Taxol and its analogs.The Journal of biological chemistry · 2020Article
- A Novel Hydroxylation Step in the Taxane Biosynthetic Pathway: A New Approach to Paclitaxel Production by Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Article
- Medically Useful Plant Terpenoids: Biosynthesis, Occurrence, and Mechanism of Action.Molecules (Basel, Switzerland) · 2019Review
- Perfluorodecalins and Hexenol as Inducers of Secondary Metabolism inFrontiers in plant science · 2018Article
- CYP79 P450 monooxygenases in gymnosperms: CYP79A118 is associated with the formation of taxiphyllin in Taxus baccata.Plant molecular biology · 2017Article
- Improving 10-deacetylbaccatin III-10-β-O-acetyltransferase catalytic fitness for Taxol production.Nature communications · 2017Article
- Tomato UDP-Glucose Sterol Glycosyltransferases: A Family of Developmental and Stress Regulated Genes that Encode Cytosolic and Membrane-Associated Forms of the Enzyme.Frontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of their biosynthesis remain unknown. In this work, a genomewide expression analysis of jasmonate-elicited Taxus baccata cell cultures by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) indicated a correlation between an extensive elicitor-induced genetic reprogramming and increased taxane production in the targeted cultures. Subsequent in silico analysis allowed us to identify 15 genes with a jasmonate-induced differential expression as putative candidates for genes encoding enzymes involved in five unknown steps of taxane biosynthesis. Among them, the TB768 gene showed a strong homology, including a very similar predicted 3D structure, with other genes previously reported to encode acyl-CoA ligases, thus suggesting a role in the formation of the taxol lateral chain. Functional analysis confirmed that the TB768 gene encodes an acyl-CoA ligase that localizes to the cytoplasm and is able to convert β-phenylalanine, as well as coumaric acid, into their respective derivative CoA esters. β-phenylalanyl-CoA is attached to baccatin III in one of the last steps of the taxol biosynthetic pathway. The identification of this gene will contribute to the establishment of sustainable taxol production systems through metabolic engineering or synthetic biology approaches.
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