ArticleMolecular microbiology2015
Carbon catabolite repression by seryl phosphorylated HPr is essential to Streptococcus pneumoniae in carbohydrate-rich environments.
Article in Molecular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- Strategies and applications for alleviating carbon catabolite repression.Synthetic and systems biotechnology · 2027Review
- Evaluation of serine protein kinase HPrK as an antibacterial target in gram-positive bacteria and mycoplasmas.Microbiology spectrum · 2026Article
- Glucose affects capsular polysaccharides synthesis via CcpA and HPr in Streptococcus pneumoniae.Journal of microbiology (Seoul, Korea) · 2025Article
- Systematic analysis of the glucose-PTS inApplied and environmental microbiology · 2025Article
- Glycerol metabolism contributes to competition by oral streptococci through production of hydrogen peroxide.Journal of bacteriology · 2024Article
- Article
- Pn-AqpC-Mediated Fermentation Pattern Coordination with the Two-Component System 07 Regulates Host N-Glycan Degradation of Streptococcus pneumoniae.Microbiology spectrum · 2022Article
- Spontaneous Mutants of Streptococcus sanguinis with Defects in the Glucose-Phosphotransferase System Show Enhanced Post-Exponential-Phase Fitness.Journal of bacteriology · 2021Article
- Genome-wide analysis of in vivo CcpA binding with and without its key co-factor HPr in the major human pathogen group A Streptococcus.Molecular microbiology · 2021Article
- Site-Specific Mutations of GalR Affect Galactose Metabolism in Streptococcus pneumoniae.Journal of bacteriology · 2020Article
- Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae.Communications biology · 2020Article
- Phosphotransferase System Uptake and Metabolism of the β-Glucoside Salicin Impact Group A Streptococcal Bloodstream Survival and Soft Tissue Infection.Infection and immunity · 2020Article
- iDS372, a Phenotypically Reconciled Model for the Metabolism ofFrontiers in microbiology · 2019Article
- Biology of Oral Streptococci.Microbiology spectrum · 2018Review
- ManLMN is a glucose transporter and central metabolic regulator in Streptococcus pneumoniae.Molecular microbiology · 2016Article
- Transcription of Sialic Acid Catabolism Genes in Corynebacterium glutamicum Is Subject to Catabolite Repression and Control by the Transcriptional Repressor NanR.Journal of bacteriology · 2016Article
- Transcriptional and metabolic effects of glucose on Streptococcus pneumoniae sugar metabolism.Frontiers in microbiology · 2015Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Carbon catabolite repression (CCR) is a regulatory phenomenon implemented by bacteria to hierarchically organize carbohydrate utilization in order to achieve maximal growth. CCR is likely of great importance to Streptococcus pneumoniae because the human host sites inhabited by this pathogen represent complex carbohydrate environments. In this species, inactivation of the prototypical Gram-positive CCR master regulator, ccpA, attenuates virulence in mice but does not relieve CCR of most metabolic enzymes, suggesting CcpA-independent CCR mechanisms predominate. Here we show the activities of three transcriptional regulators constitute the majority of transcriptional CCR of galactose metabolism operons. We determined seryl-phosphorylated histidine phosphocarrier protein (HPr-Ser∼P)-mediated regulation is a major CCR mechanism and an essential activity in the pneumococcus, as an HPr point mutation abolishing HPrK/P-dependent phosphorylation was not tolerated nor was deletion of hprk/p. The HPr-Ser∼P phosphomimetic mutant HPr S46D had reduced phosphotransferase system transport rates and limited induction of CCR-repressed genes. These results support a model of pneumococcal CCR in which HPr-Ser∼P directly affects the activity of CcpA while indirectly affecting the activity of pathway-specific transactional regulators. This report describes the first CcpA-independent CCR mechanism identified in the pneumococcus and the first example of lethality from loss of HPr-Ser∼P-mediated CCR in any species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.