ArticleIn vitro cellular & developmental biology. Animal2015
In vitro evaluation of different protocols for the induction of mesenchymal stem cells to insulin-producing cells.
Article in In vitro cellular & developmental biology. Animal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 17 citations in OpenAlex.
- Human umbilical cord mesenchymal stem cells in diabetes mellitus and its complications: applications and research advances.International journal of medical sciences · 2023Review
- Benchtop Isolation and Characterisation of Small Extracellular Vesicles from Human Mesenchymal Stem Cells.Molecular biotechnology · 2021Review
- Insulin-Producing Cell Transplantation Platform for Veterinary Practice.Frontiers in veterinary science · 2020Review
- Regulation of somatostatin expression by vitamin D3 and valproic acid in human adipose-derived mesenchymal stem cells.Stem cell research & therapy · 2019Article
- Suspension Culture Alters Insulin Secretion in Induced Human Umbilical Cord Matrix-Derived Mesenchymal Cells.Cell journal · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cells therapy is a new promising approach for diabetes mellitus (DM) treatment, but the insulin secretion rate in differentiated cells is low when compared with pancreas beta cells embedded in Langerhans islets. In this study, we evaluated different protocols of insulin secretion to achieve the most appropriate protocol for in vitro insulin secretion. We differentiated human umbilical cord matrix-derived mesenchymal cells (hUCMs) into insulin-producing cell (IPC) by the aim of three previously reported protocols and a modified protocol. The insulin content was analyzed through gene expression and immunocytochemistry (IHC). Dithizone (DTZ) staining was done for identification of islet-like structures. Insulin and C peptide secretion was measured by chemiluminesence immunoassay (CLIA) and enzyme immunoassay (EIA) as well. Reverse transcription-PCR (RT-PCR) showed efficient expression of insulin genes in all the study groups. IHC analysis showed higher expression of insulin and proinsulin proteins in the modified protocol. DTZ staining exhibited variable islet-like clusters in the different protocols except control. This finding was confirmed by the higher response to glucose challenge test in this group. A modified protocol using an intermediate step that makes the cells vulnerable to nestin production in combination with inducing agent results in the higher differentiation of stem cells into insulin-producing cells and more insulin secretion in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.