ArticleAnalytical chemistry2015
High-speed multiparameter photophysical analyses of fluorophore libraries.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- Dark-State-Mediated Photobleaching in mCherry-Based Red Fluorescent Proteins.The journal of physical chemistry letters · 2026Article
- Spatial Encoding with Amplitude Modulation in Serial Flow Cytometry.Sensors (Basel, Switzerland) · 2026Article
- A highly photostable monomeric red fluorescent protein for dual-color 3D STED and time-lapse 3D SIM imaging.Nature methods · 2026Article
- StayGold variants for molecular fusion and membrane-targeting applications.Nature methods · 2024Article
- A Combination of Library Screening and Rational Mutagenesis Expands the Available Color Palette of the Smallest Fluorogen-Activating Protein Tag nanoFAST.International journal of molecular sciences · 2024Article
- A highly photostable and bright green fluorescent protein.Nature biotechnology · 2022Article
- In vitro optoacoustic flow cytometry with light scattering referencing.Scientific reports · 2021Article
- Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity.Communications biology · 2020Article
- Quantifying Acute Fuel and Respiration Dependent pH Homeostasis in Live Cells Using the mCherryTYG Mutant as a Fluorescence Lifetime Sensor.Analytical chemistry · 2019Article
- Directed evolution of excited state lifetime and brightness in FusionRed using a microfluidic sorter.Integrative biology : quantitative biosciences from nano to macro · 2018Article
- A guide to choosing fluorescent protein combinations for flow cytometric analysis based on spectral overlap.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Article
- The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.Trends in biochemical sciences · 2017Review
- Droplet Microfluidic Flow Cytometer For Sorting On Transient Cellular Responses Of Genetically-Encoded Sensors.Analytical chemistry · 2017Article
- Microfluidic System for In-Flow Reversible Photoswitching of Near-Infrared Fluorescent Proteins.Analytical chemistry · 2016Article
- Microfluidic techniques for high throughput single cell analysis.Current opinion in biotechnology · 2016Review
- Phasor plotting with frequency-domain flow cytometry.Optics express · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
There is a critical need for high-speed multiparameter photophysical measurements of large libraries of fluorescent probe variants for imaging and biosensor development. We present a microfluidic flow cytometer that rapidly assays 10(4)-10(5) member cell-based fluorophore libraries, simultaneously measuring fluorescence lifetime and photobleaching. Together, these photophysical characteristics determine imaging performance. We demonstrate the ability to resolve the diverse photophysical characteristics of different library types and the ability to identify rare populations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.