ArticleeLife2015
A novel isoform of MAP4 organises the paraxial microtubule array required for muscle cell differentiation.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 52 citations in OpenAlex.
- Mechanisms of cortical microtubule organization in epidermal keratinocytes.Cellular and molecular life sciences : CMLS · 2025Article
- SH3KBP1 promotes skeletal myofiber formation and functionality through ER/SR architecture integrity.EMBO reports · 2025Article
- Molecular composition of skeletal muscle in infants and adults: a comparative proteomic and transcriptomic study.Scientific reports · 2024Article
- Microtubule-associated protein MAP7 promotes tubulin posttranslational modifications and cargo transport to enable osmotic adaptation.Developmental cell · 2024Article
- Macromolecular Crowding Tailors the Microtubule Cytoskeleton Through Tubulin Modifications and Microtubule-Associated Proteins.bioRxiv : the preprint server for biology · 2023Article
- miR-103-3p Regulates the Differentiation and Autophagy of Myoblasts by Targeting MAP4.International journal of molecular sciences · 2023Article
- Insights into Cell-Specific Functions of Microtubules in Skeletal Muscle Development and Homeostasis.International journal of molecular sciences · 2023Review
- Myristic acid selectively augments β-tubulin levels in C2C12 myotubes via diacylglycerol kinase δ.FEBS open bio · 2022Article
- The prophase oocyte nucleus is a homeostatic G-actin buffer.Journal of cell science · 2022Article
- Article
- Mini-review: Microtubule sliding in neurons.Neuroscience letters · 2021Review
- CAPN6 in disease: An emerging therapeutic target (Review).International journal of molecular medicine · 2020Review
- Rapid, redox-mediated mechanical susceptibility of the cortical microtubule lattice in skeletal muscle.Redox biology · 2020Article
- Longitudinal serum biomarker screening identifies malate dehydrogenase 2 as candidate prognostic biomarker for Duchenne muscular dystrophy.Journal of cachexia, sarcopenia and muscle · 2020Article
- Supporting the heart: Functions of the cardiomyocyte's non-sarcomeric cytoskeleton.Journal of molecular and cellular cardiology · 2019Review
- WIPI1 is a conserved mediator of right ventricular failure.JCI insight · 2019Article
- Survey of allele specific expression in bovine muscle.Scientific reports · 2019Article
- Actin-microtubule crosstalk in cell biology.Nature reviews. Molecular cell biology · 2019Review
- Requirement of Pitx2 for skeletal muscle homeostasis.Developmental biology · 2019Article
- Measuring microtubule dynamics.Essays in biochemistry · 2018Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The microtubule cytoskeleton is critical for muscle cell differentiation and undergoes reorganisation into an array of paraxial microtubules, which serves as template for contractile sarcomere formation. In this study, we identify a previously uncharacterised isoform of microtubule-associated protein MAP4, oMAP4, as a microtubule organising factor that is crucial for myogenesis. We show that oMAP4 is expressed upon muscle cell differentiation and is the only MAP4 isoform essential for normal progression of the myogenic differentiation programme. Depletion of oMAP4 impairs cell elongation and cell-cell fusion. Most notably, oMAP4 is required for paraxial microtubule organisation in muscle cells and prevents dynein- and kinesin-driven microtubule-microtubule sliding. Purified oMAP4 aligns dynamic microtubules into antiparallel bundles that withstand motor forces in vitro. We propose a model in which the cooperation of dynein-mediated microtubule transport and oMAP4-mediated zippering of microtubules drives formation of a paraxial microtubule array that provides critical support for the polarisation and elongation of myotubes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.