ArticleNature communications2015
Designer diatom episomes delivered by bacterial conjugation.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 137 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
137 citing papers in PubMed, 319 citations in OpenAlex.
- PHYCUT: Scalable Multiplex CRISPR/Cas9 Editing for Genome Engineering in the DiatomACS synthetic biology · 2026Article
- Characterization of fucoxanthin-deficient strains in the haptophyte Tisochrysis lutea induced by heavy-ion beam irradiation.Plant & cell physiology · 2026Article
- Tryptophanol enhances nitrogen assimilation in marine diatoms.Nature communications · 2026Article
- Real-Time Tracking of Intracellular Prenyl Phosphate Pools in the Marine DiatomACS synthetic biology · 2026Article
- Review
- A modular synthetic biology toolkit unlocks metabolic engineering of the industrially relevant alga Nannochloropsis.Advanced biotechnology · 2026Article
- Breaking the cell wall for efficient DNA delivery to diatoms.Nature communications · 2026Article
- Double-strand break-free and transgene-free genome editing in the microalga Nannochloropsis oceanica using removable vectors containing the CRISPR base editing system.Scientific reports · 2025Article
- Pan-microalgal dark proteome mapping via interpretable deep learning and synthetic chimeras.Patterns (New York, N.Y.) · 2025Article
- The marine diatom Phaeodactylum tricornutum as a versatile bioproduction chassis: Current progress, challenges, and perspectives.Plant communications · 2025Review
- Creating an Improved Diatoxanthin Production Line by Knocking OutMarine drugs · 2025Article
- Both major xanthophyll cycles present in nature promote nonphotochemical quenching in a model diatom.Plant physiology · 2025Article
- Boosting energy metabolism and biosynthesis in diverse organisms by a common bacterial salvage lipoylation protein.Nature communications · 2025Article
- Novel centromeric plasmid for stable extrachromosomal gene expression in Aurantiochytrium limacinum.Applied microbiology and biotechnology · 2025Article
- Remodeling of the terpenoid metabolism during prolonged phosphate depletion in the marine diatom Phaeodactylum tricornutum.Journal of phycology · 2025Article
- The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025Review
- Characterization of the VOC Promoter That Is Active Under Low-Salinity Conditions in the DiatomMarine drugs · 2025Article
- XanthoMoClo─A Robust Modular Cloning Genetic Toolkit for the GeneraACS synthetic biology · 2025Article
- Impact of heterologous expression of Cannabis sativa tetraketide synthase on Phaeodactylum tricornutum metabolic profile.Biotechnology for biofuels and bioproducts · 2025Article
- Review
77 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
20 authors at 3 institutions in 1 country.
Funding
Abstract
Eukaryotic microalgae hold great promise for the bioproduction of fuels and higher value chemicals. However, compared with model genetic organisms such as Escherichia coli and Saccharomyces cerevisiae, characterization of the complex biology and biochemistry of algae and strain improvement has been hampered by the inefficient genetic tools. To date, many algal species are transformable only via particle bombardment, and the introduced DNA is integrated randomly into the nuclear genome. Here we describe the first nuclear episomal vector for diatoms and a plasmid delivery method via conjugation from Escherichia coli to the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana. We identify a yeast-derived sequence that enables stable episome replication in these diatoms even in the absence of antibiotic selection and show that episomes are maintained as closed circles at copy number equivalent to native chromosomes. This highly efficient genetic system facilitates high-throughput functional characterization of algal genes and accelerates molecular phytoplankton research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.