ArticleBiotechnology for biofuels2015
Detoxification of 5-hydroxymethylfurfural by the Pleurotus ostreatus lignolytic enzymes aryl alcohol oxidase and dehydrogenase.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 77 citations in OpenAlex.
- Advance biocatalyst for the green conversion of 5-hydroxymethylfurfural to a biopolymer precursor 2,5-furandicarboxylic acid.Biotechnology for biofuels and bioproducts · 2025Article
- Transcriptome analysis of Aspergillus oryzae RIB40 under chemical stress reveals mechanisms of adaptation to fungistatic compounds of lignocellulosic side streams.Biotechnology for biofuels and bioproducts · 2025Article
- Hydrated lime promoted the polysaccharide content and affected the transcriptomes ofFrontiers in microbiology · 2023Article
- Advancement of biorefinery-derived platform chemicals from macroalgae: a perspective for bioethanol and lactic acid.Biomass conversion and biorefinery · 2022Review
- A LignocellulolyticJournal of fungi (Basel, Switzerland) · 2021Article
- Bioengineering advancements, innovations and challenges on green synthesis of 2, 5-furan dicarboxylic acid.Bioengineered · 2020Review
- Securing a furan-based biorefinery: disclosing the genetic basis of the degradation of hydroxymethylfurfural and its derivatives in the model fungus Aspergillus nidulans.Microbial biotechnology · 2020Article
- Enzymatic removal of inhibitory compounds from lignocellulosic hydrolysates for biomass to bioproducts applications.World journal of microbiology & biotechnology · 2020Review
- Crosslinked Enzyme Aggregates (CLEAs) of Laccases fromMolecules (Basel, Switzerland) · 2020Article
- Enzymatic conversion reactions of 5-hydroxymethylfurfural (HMF) to bio-basedBiotechnology for biofuels · 2020Article
- Manipulating the Expression of Small Secreted Protein 1 (Ssp1) Alters Patterns of Development and Metabolism in the White-Rot FungusApplied and environmental microbiology · 2019Article
- Identification and manipulation ofBiotechnology for biofuels · 2019Article
- An Overview of Genes FromFrontiers in microbiology · 2019Article
- Enzymatic Preparation of 2,5-Furandicarboxylic Acid (FDCA)-A Substitute of Terephthalic Acid-By the Joined Action of Three Fungal Enzymes.Microorganisms · 2018Article
- Self-sustained enzymatic cascade for the production of 2,5-furandicarboxylic acid from 5-methoxymethylfurfural.Biotechnology for biofuels · 2018Article
- Effects ofBiotechnology for biofuels · 2018Article
- A role for small secreted proteins (SSPs) in a saprophytic fungal lifestyle: Ligninolytic enzyme regulation in Pleurotus ostreatus.Scientific reports · 2017Article
- Differential Proteomic Profiles ofFrontiers in microbiology · 2017Article
- TheBiotechnology for biofuels · 2017Article
- Localizing gene regulation reveals a staggered wood decay mechanism for the brown rot fungus Postia placenta.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCurrent large-scale pretreatment processes for lignocellulosic biomass are generally accompanied by the formation of toxic degradation products, such as 5-hydroxymethylfurfural (HMF), which inhibit cellulolytic enzymes and fermentation by ethanol-producing yeast. Overcoming these toxic effects is a key technical barrier in the biochemical conversion of plant biomass to biofuels. Pleurotus ostreatus, a white-rot fungus, can efficiently degrade lignocellulose. In this study, we analyzed the ability of P. ostreatus to tolerate and metabolize HMF and investigated relevant molecular pathways associated with these processes.
resultsP. ostreatus was capable to metabolize and detoxify HMF 30 mM within 48 h, converting it into 2,5-bis-hydroxymethylfuran (HMF alcohol) and 2,5-furandicarboxylic acid (FDCA), which subsequently allowed the normal yeast growth in amended media. We show that two enzymes groups, which belong to the ligninolytic system, aryl-alcohol oxidases and a dehydrogenase, are involved in this process. HMF induced the transcription and production of these enzymes and was accompanied by an increase in activity levels. We also demonstrate that following the induction of these enzymes, HMF could be metabolized in vitro.
conclusionsAryl-alcohol oxidase and dehydrogenase gene family members are part of the transcriptional and subsequent translational response to HMF exposure in P. ostreatus and are involved in HMF transformation. Based on our data, we propose that these enzymatic capacities of P. ostreatus either be integrated in biomass pretreatment or the genes encoding these enzymes may function to detoxify HMF via heterologous expression in fermentation organisms, such as Saccharomyces cerevisiae.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.