ArticleThe Journal of cell biology2015
Cell motion predicts human epidermal stemness.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Coarse-graining analysis for non-invasive, label-free, and quantitative evaluation of undulating motion in cultured oral mucosa epithelial cell sheets.Regenerative therapy · 2026Article
- Topology-guided polar ordering of collective cell migration.Science advances · 2024Article
- Article
- EGFR-mediated epidermal stem cell motility drives skin regeneration through COL17A1 proteolysis.The Journal of cell biology · 2021Article
- Label-free quality control and identification of human keratinocyte stem cells by deep learning-based automated cell tracking.Stem cells (Dayton, Ohio) · 2021Article
- Principles and mechanisms of asymmetric cell division.Development (Cambridge, England) · 2020Review
- Cell jamming, stratification and p63 expression in cultivated human corneal epithelial cell sheets.Scientific reports · 2020Article
- Evaluation of the proliferative potential of skin keratinocytes and fibroblasts isolated from critical limb ischemia patients.Regenerative therapy · 2020Article
- Epidermal Stem Cells in Hair Follicle Cycling and Skin Regeneration: A View From the Perspective of Inflammation.Frontiers in cell and developmental biology · 2020Review
- Automated collective motion analysis validates human keratinocyte stem cell cultures.Scientific reports · 2019Article
- In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-Like Characteristics.Scientific reports · 2019Article
- IRF2 is a master regulator of human keratinocyte stem cell fate.Nature communications · 2019Article
- Article
- Patterning of human epidermal stem cells on undulating elastomer substrates reflects differences in cell stiffness.Acta biomaterialia · 2019Article
- Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture.Nature communications · 2018Article
- Coordinated collective migration and asymmetric cell division in confluent human keratinocytes without wounding.Nature communications · 2018Article
- Stem Cell Properties of Normal Human Keratinocytes Determine Transformation Responses to Human Papillomavirus 16 DNA.Journal of virology · 2018Article
- Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond.EMBO molecular medicine · 2018Review
- PFKFB3, a Direct Target of p63, Is Required for Proliferation and Inhibits Differentiation in Epidermal Keratinocytes.The Journal of investigative dermatology · 2017Article
- Tuning Collective Cell Migration by Cell-Cell Junction Regulation.Cold Spring Harbor perspectives in biology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Image-based identification of cultured stem cells and noninvasive evaluation of their proliferative capacity advance cell therapy and stem cell research. Here we demonstrate that human keratinocyte stem cells can be identified in situ by analyzing cell motion during their cultivation. Modeling experiments suggested that the clonal type of cultured human clonogenic keratinocytes can be efficiently determined by analysis of early cell movement. Image analysis experiments demonstrated that keratinocyte stem cells indeed display a unique rotational movement that can be identified as early as the two-cell stage colony. We also demonstrate that α6 integrin is required for both rotational and collective cell motion. Our experiments provide, for the first time, strong evidence that cell motion and epidermal stemness are linked. We conclude that early identification of human keratinocyte stem cells by image analysis of cell movement is a valid parameter for quality control of cultured keratinocytes for transplantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.