ArticleThe International journal of developmental biology2014
Optogenetics in Developmental Biology: using light to control ion flux-dependent signals in Xenopus embryos.
Article in The International journal of developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed.
- Assessing circuit function in the developingFrontiers in behavioral neuroscience · 2026Review
- Collective intelligence: A unifying concept for integrating biology across scales and substrates.Communications biology · 2024Review
- Article
- The scaling of goals from cellular to anatomical homeostasis: an evolutionary simulation, experiment and analysis.Interface focus · 2023Article
- Cellular signaling pathways as plastic, proto-cognitive systems: Implications for biomedicine.Patterns (New York, N.Y.) · 2023Review
- There's Plenty of Room Right Here: Biological Systems as Evolved, Overloaded, Multi-Scale Machines.Biomimetics (Basel, Switzerland) · 2023Article
- Information Theory as an Experimental Tool for Integrating Disparate Biophysical Signaling Modules.International journal of molecular sciences · 2022Article
- Technological Approach to Mind Everywhere: An Experimentally-Grounded Framework for Understanding Diverse Bodies and Minds.Frontiers in systems neuroscience · 2022Article
- Unveiling the morphogenetic code: A new path at the intersection of physical energies and chemical signaling.World journal of stem cells · 2021Review
- Synthetic living machines: A new window on life.iScience · 2021Review
- Reverse and Forward Engineering Multicellular Structures with Optogenetics.Current opinion in biomedical engineering · 2020Article
- Toward Decoding Bioelectric Events in Xenopus Embryogenesis: New Methodology for Tracking Interplay Between Calcium and Resting Potentials In Vivo.Journal of molecular biology · 2020Article
- Modulation of Cellular Reactivity for Enhanced Cell-Based Biosensing.Analytical chemistry · 2020Article
- Review
- Preventing Ethanol-Induced Brain and Eye Morphology Defects Using Optogenetics.Bioelectricity · 2019Article
- Repurposing Protein Degradation for Optogenetic Modulation of Protein Activities.ACS synthetic biology · 2019Article
- Planarian regeneration as a model of anatomical homeostasis: Recent progress in biophysical and computational approaches.Seminars in cell & developmental biology · 2019Review
- Bioelectrical control of positional information in development and regeneration: A review of conceptual and computational advances.Progress in biophysics and molecular biology · 2018Review
- The bioelectric code: An ancient computational medium for dynamic control of growth and form.Bio Systems · 2018Review
- The brain is required for normal muscle and nerve patterning during early Xenopus development.Nature communications · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Developmental bioelectricity, electrical signaling among non-excitable cells, is now known to regulate proliferation, apoptosis, gene expression, and patterning during development. The extraordinary temporal and spatial resolution offered by optogenetics could revolutionize the study of bioelectricity the same way it has revolutionized neuroscience. There is, however, no guide to adapting optogenetics to patterning systems. To fill this gap, we used optogenetic reagents, both proteins and photochemical switches, to vary steady-state bioelectrical properties of non-spiking embryonic cells in Xenopus laevis. We injected mRNA for various proteins, including Channelrhodopsins and Archaerhodopsin, into 1-8 cell embryos, or soaked embryos in media containing photochemical switches, then examined the effect of light and dark on membrane voltage (Vmem) using both electrodes and fluorescent membrane voltage reporters. We also scored tadpoles for known effects of varying Vmem, including left-right asymmetry disruption, hyperpigmentation, and craniofacial phenotypes. The majority of reagents we tested caused a significant increase in the percentage of light-exposed tadpoles showing relevant phenotypes; however, the majority of reagents also induced phenotypes in controls kept in the dark. Experiments on this "dark phenotype" yielded evidence that the direction of ion flux via common optogenetic reagents may be reversed, or unpredictable in non-neural cells. When used in combination with rigorous controls, optogenetics can be a powerful tool for investigating ion-flux based signaling in non-excitable systems. Nonetheless, it is crucial that new reagents be designed with these non-neural cell types in mind.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.