Evidence map›Paper›PMID 25895995›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2015

Silver nanoclusters for RNA nanotechnology: steps towards visualization and tracking of RNA nanoparticle assemblies.

Kirill A Afonin, Danielle Schultz, Luc Jaeger, Elisabeth Gwinn, Bruce A Shapiro

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kirill A AfoninCenter for Cancer Research Nanobiology Program, National Cancer Institute, Frederick, MD, 21702, USA.
Danielle Schultz
Luc Jaeger
Elisabeth Gwinn
Bruce A Shapiro

Funding

Computational RNA NanodesignZIABC011061 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI SHAPIRO, BRUCE · 2009 to 2021
$12.3M
Exploring the RNA syntax for engineering therapeutic RNA-based nano-factoriesR01GM079604 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI JAEGER, LUC · 2007 to 2011
$1.7M
Intramural NIH HHS ZIA BC011061NIGMS NIH HHS R01 GM079604NIGMS NIH HHS R01GM-079604
6 · The paper itself

Abstract

The growing interest in designing functionalized, RNA-based nanoparticles (NPs) for applications such as cancer therapeutics requires simple, efficient assembly assays. Common methods for tracking RNA assemblies such as native polyacrylamide gels and atomic force microscopy are often time-intensive and, therefore, undesirable. Here we describe a technique for rapid analysis of RNA NP assembly stages using the formation of fluorescent silver nanoclusters (Ag NCs). This method exploits the single-stranded specificity and sequence dependence of Ag NC formation to produce unique optical readouts for each stage of RNA NP assembly, obtained readily after synthesis.

Indexed as

HumansMetal NanoparticlesMicroscopy, Atomic ForceNanotechnologyRNASilverRNASilver

Identifiers

PMID25895995
PMCPMC6345514

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.