ArticleInfection and immunity2015
The Serine Protease Autotransporter Pic Modulates Citrobacter rodentium Pathogenesis and Its Innate Recognition by the Host.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- The growth and pathogenesis ofInfection and immunity · 2026Article
- Maternal antibodies against the immunoglobulin M-degrading enzyme of Streptococcus suis, IdeVeterinary research · 2026Article
- Defining enteric bacterial pathogenesis using organoids:Gut microbes · 2025Article
- Cathelicidin regulates goblet cell mucus secretion and mucus-associated proteins inGut microbes · 2025Article
- Bacteriophage Mu contamination impacts interbacterial competition.Infection and immunity · 2025Article
- Sialic acid plays a pivotal role in licensingProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Novel organisation and regulation of theVirulence · 2022Article
- Cinnamaldehyde Increases the Survival of Mice Submitted to Sepsis Induced by Extraintestinal PathogenicAntibiotics (Basel, Switzerland) · 2022Article
- Phylogenetic Classification and Functional Review of Autotransporters.Frontiers in immunology · 2022Review
- Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli.Scientific reports · 2020Article
- The Serine Protease Autotransporters TagB, TagC, and Sha from Extraintestinal PathogenicInternational journal of molecular sciences · 2020Article
- Pic-ProducingInternational journal of molecular sciences · 2020Article
- Pic Protein From EnteroaggregativeFrontiers in immunology · 2020Article
- Serine Protease Autotransporters of theMicroorganisms · 2019Review
- Frontline defenders: goblet cell mediators dictate host-microbe interactions in the intestinal tract during health and disease.American journal of physiology. Gastrointestinal and liver physiology · 2018Review
- Recognition of protein-linked glycans as a determinant of peptidase activity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Immunological aspects of intestinal mucus and mucins.Nature reviews. Immunology · 2016Review
- The serine protease Pic as a virulence factor of atypical enteropathogenic Escherichia coli.Gut microbes · 2016Article
- Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Bacterial pathogens produce a number of autotransporters that possess diverse functions. These include the family of serine protease autotransporters of Enterobacteriaceae (SPATEs) produced by enteric pathogens such as Shigella flexneri and enteroaggregative Escherichia coli. Of these SPATEs, one termed "protein involved in colonization," or Pic, has been shown to possess mucinase activity in vitro, but to date, its role in in vivo enteric pathogenesis is unknown. Testing a pic null (ΔpicC) mutant in Citrobacter rodentium, a natural mouse pathogen, found that the C. rodentium ΔpicC strain was impaired in its ability to degrade mucin in vitro compared to the wild type. Upon infection of mice, the ΔpicC mutant exhibited a hypervirulent phenotype with dramatically heavier pathogen burdens found in intestinal crypts. ΔpicC mutant-infected mice suffered greater barrier disruption and more severe colitis and weight loss, necessitating their euthanization between 10 and 14 days postinfection. Notably, the virulence of the ΔpicC mutant was normalized when the picC gene was restored; however, a PicC point mutant causing loss of mucinase activity did not replicate the ΔpicC phenotype. Exploring other aspects of PicC function, the ΔpicC mutant was found to aggregate to higher levels in vivo than wild-type C. rodentium. Moreover, unlike the wild type, the C. rodentium ΔpicC mutant had a red, dry, and rough (RDAR) morphology in vitro and showed increased activation of the innate receptor Toll-like receptor 2 (TLR2). Interestingly, the C. rodentium ΔpicC mutant caused a degree of pathology similar to that of wild-type C. rodentium when infecting TLR2-deficient mice, showing that despite its mucinase activity, PicC's major role in vivo may be to limit C. rodentium's stimulation of the host's innate immune system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.