ArticlePlant cell reports2015
Downregulation of transcription factor aflR in Aspergillus flavus confers reduction to aflatoxin accumulation in transgenic maize with alteration of host plant architecture.
Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.
- Resistance of Transgenic Maize Cultivars to Mycotoxin Production-Systematic Review and Meta-Analysis.Toxins · 2024Pooled it
- Molecular Identification and RNA-Based Management of Fungal Plant Pathogens: From PCR to CRISPR/Cas9.International journal of molecular sciences · 2026Review
- Review
- Harnessing double-stranded RNA (dsRNA): a sustainable approach to pest management.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Host-Induced Gene Silencing of theToxins · 2024Article
- A gene regulating ergot alkaloid biosynthesis inApplied and environmental microbiology · 2024Article
- TheJournal of fungi (Basel, Switzerland) · 2024Article
- RNAi-Based Approaches to Control Mycotoxin Producers: Challenges and Perspectives.Journal of fungi (Basel, Switzerland) · 2024Review
- Aflatoxins Contamination in Feed Commodities: From Occurrence and Toxicity to Recent Advances in Analytical Methods and Detoxification.Microorganisms · 2023Review
- Multiplexed Host-Induced Gene Silencing ofToxins · 2023Article
- Targeting theFrontiers in plant science · 2023Article
- Small RNA-based plant protection against diseases.Frontiers in plant science · 2022Review
- Advanced mycotoxin control and decontamination techniques in view of an increased aflatoxin risk in Europe due to climate change.Frontiers in microbiology · 2022Review
- Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize.BMC biotechnology · 2021Article
- Resistance to Aflatoxin Accumulation in Maize Mediated by Host-Induced Silencing of theJournal of fungi (Basel, Switzerland) · 2021Article
- Aflatoxins: History, Significant Milestones, Recent Data on Their Toxicity and Ways to Mitigation.Toxins · 2021Review
- The PHD transcription factor Cti6 is involved in the fungal colonization and aflatoxin B1 biological synthesis of Aspergillus flavus.IMA fungus · 2021Article
- RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges.Plants (Basel, Switzerland) · 2021Article
- Targeted Delivery of Gene Silencing in Fungi Using Genetically Engineered Bacteria.Journal of fungi (Basel, Switzerland) · 2021Article
- Host Induced Gene Silencing TargetingFrontiers in microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageWe report success of host-induced gene silencing in downregulation of aflatoxin biosynthesis in Aspergillus flavus infecting maize transformed with a hairpin construct targeting transcription factor aflR. Infestation of crops by aflatoxin-producing fungi results in economic losses as well as negative human and animal health effects. Currently, the control strategies against aflatoxin accumulation are not effective to the small holder farming systems in Africa and this has led to widespread aflatoxin exposure especially in rural populations of sub-Saharan Africa that rely on maize as a staple food crop. A recent strategy called host-induced gene silencing holds great potential for developing aflatoxin-resistant plant germplasm for the African context where farmers are unable to make further investments other than access to the germplasm. We transformed maize with a hairpin construct targeting the aflatoxin biosynthesis transcription factor aflR. The developed transgenic maize were challenged with an aflatoxigenic Aspergillus flavus strain from Eastern Kenya, a region endemic to aflatoxin outbreaks. Our results indicated that aflR was downregulated in A. flavus colonizing transgenic maize. Further, maize kernels from transgenic plants accumulated significantly lower levels of aflatoxins (14-fold) than those from wild type plants. Interestingly, we observed that our silencing cassette caused stunting and reduced kernel placement in the transgenic maize. This could have been due to "off-target" silencing of unintended genes in transformed plants by aflR siRNAs. Overall, this work indicates that host-induced gene silencing has potential in developing aflatoxin-resistant germplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.