ArticleParasitology research2015
Development of a multiplex PCR assay for detection and discrimination of Theileria annulata and Theileria sergenti in cattle.
Article in Parasitology research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 51 citations in OpenAlex.
- Development of hexaplex PCR assay for the detection and characterization common tick-borne pathogens in dogs, and analysis of risk factors.Veterinary and animal science · 2026Article
- Prevalence and molecular detection ofParasite epidemiology and control · 2026Article
- Theileria annulata diagnostics: past, present and future.Tropical animal health and production · 2026Review
- Molecular epidemiology and phylogenetic relationships of Theileria spp. in cattle from Marathwada region of Maharashtra state, India.Tropical animal health and production · 2025Article
- Molecular characterization of Rhipicephalus microplus and tick-borne pathogens in cattle in Nepal.Veterinary research communications · 2025Article
- SNP-based molecular diagnostic platform: rapid single-step identification of Theileria annulata and its buparvaquone-resistant strains.Parasites & vectors · 2025Article
- An epidemiological survey of bovine piroplasmosis in Kashgar, Xinjiang, China.Parasitology research · 2024Article
- Simultaneous and rapid detection of avian respiratory diseases of small poultry using multiplex reverse transcription-Polymerase Chain Reaction assay.Poultry science · 2023Article
- Multiplex PCR for rapid differential diagnosis of co-prevalent species of Theileria (Theileria annulata and Theileria orientalis) in cattle.Parasitology research · 2023Article
- Theileria annulata SVSP455 interacts with host HSP60.Parasites & vectors · 2022Article
- Establishment and application of a qPCR diagnostic method for Theileria annulata.Parasitology research · 2022Article
- Seroprevalence and risk factors of tropical theileriosis in smallholder asymptomatic large ruminants in Egypt.Frontiers in veterinary science · 2022Article
- Article
- Clinical and Pathological Studies on Cattle Experimentally Infected withPathogens (Basel, Switzerland) · 2020Article
- Clinical Pathology, Immunopathology and Advanced Vaccine Technology in Bovine Theileriosis: A Review.Pathogens (Basel, Switzerland) · 2020Review
- Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virus.BMC veterinary research · 2020Article
- An appraisal of oriental theileriosis and the Theileria orientalis complex, with an emphasis on diagnosis and genetic characterisation.Parasitology research · 2020Review
- Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducks.BMC veterinary research · 2019Article
- Molecular detection and genetic diversity of Theileria orientalis in cattle in China.Parasitology research · 2018Article
- Exploring the TLR and NLR signaling pathway relevant molecules induced by the Theileria annulata infection in calves.Parasitology research · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aim to construct a simple and efficient diagnostic assay for Theileria annulata and Theileria sergenti, a multiplex polymerase chain reaction (PCR) method was developed in this study. Following the alignment of the related sequences, two primer sets were designed specific targeting on T. annulata cytochrome b (COB) gene and T. sergenti internal transcribed spacer (ITS) sequences. It was found that the designed primers could react in one PCR system and generating amplifications of 818 and 393 base pair for T. sergenti and T. annulata, respectively. The standard genomic DNA of both species Theileria was serial tenfold diluted for testing the sensitivity, while specificity test confirmed both primer sets have no cross-reaction with other Theileria and Babesia species. In addition, 378 field samples were used for evaluation of the utility of the multiplex PCR assay for detection of the pathogens infection. The detection results were compared with the other two published PCR methods which targeting on T. annulata COB gene and T. sergenti major piroplasm surface protein (MPSP) gene, respectively. The developed multiplex PCR assay has similar efficient detection with COB and MPSP PCR, which indicates this multiplex PCR may be a valuable assay for the epidemiological studies for T. annulata and T. sergenti.
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