ArticleNature methods2015
A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy.
Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- A Versatile Tool to Predict and Guide RESOLFT Images Based on Photoswitching, Labelling and Optical Properties.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Two-dimensional nonlinear structured illumination microscopy with rsEGFP2.Biomedical optics express · 2025Article
- Ultrafast photophysics of a positive reversibly switchable fluorescent protein.Chemical science · 2025Article
- High contrast fluorescence polarization microscopy through double tagged photoswitchable fluorescent proteins.Npj imaging · 2025Article
- Article
- Genetically-encoded temperature indicators for thermal biology.Biophysics and physicobiology · 2025Article
- A primer to directed evolution: current methodologies and future directions.RSC chemical biology · 2023Review
- Photoswitchable Fluorescent Proteins: Mechanisms on Ultrafast Timescales.International journal of molecular sciences · 2022Review
- Technological advances in super-resolution microscopy to study cellular processes.Molecular cell · 2022Review
- Fluorescent Probes for STED Optical Nanoscopy.Nanomaterials (Basel, Switzerland) · 2021Review
- A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging.Microscopy (Oxford, England) · 2021Article
- Article
- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Fluorescent Protein-Based Indicators for Functional Super-Resolution Imaging of Biomolecular Activities in Living Cells.International journal of molecular sciences · 2019Review
- Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy.Nature communications · 2019Article
- Differential nanoscale organisation of LFA-1 modulates T-cell migration.Journal of cell science · 2019Article
- Second harmonic generation polarization microscopy as a tool for protein structure analysis.Biophysics and physicobiology · 2019Article
- Specialty probes give super-res imaging that special blink.Nature methods · 2018Article
- Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.Nature methods · 2018Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence nanoscopy has revolutionized our ability to visualize biological structures not resolvable by conventional microscopy. However, photodamage induced by intense light exposure has limited its use in live specimens. Here we describe Kohinoor, a fast-switching, positively photoswitchable fluorescent protein, and show that it has high photostability over many switching repeats. With Kohinoor, we achieved super-resolution imaging of live HeLa cells using biocompatible, ultralow laser intensity (0.004 J/cm(2)) in reversible saturable optical fluorescence transition (RESOLFT) nanoscopy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.