Evidence map›Paper›PMID 25894375›Full record

ArticleTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine2015

ShRNA-mediated knock-down of CXCR7 increases TRAIL-sensitivity in MCF-7 breast cancer cells.

Weiran Gao, Xifan Mei, Jikun Wang, Xianglin Zhang, Yajiang Yuan

Abstract read
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In one paragraph

Article in Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Weiran GaoDepartment of Oncology, The First Affiliated Hospital of Liaoning Medical University, No.2, Wuduan, Renmin Street, 121000, Jinzhou, China.
Xifan MeiDepartment of Research, Liaoning Medical University, No.40, Songpo Road, Taihe District, 121000, Jinzhou, China.
Jikun WangDepartment of Oncology, The First Affiliated Hospital of Liaoning Medical University, No.2, Wuduan, Renmin Street, 121000, Jinzhou, China.
Xianglin ZhangDepartment of Radiology, The First Affiliated Hospital of Liaoning Medical University, No.2, Wuduan, Renmin Street, 121000, Jinzhou, China.
Yajiang YuanDepartment of Orthopedic, The First Affiliated Hospital of Liaoning Medical University, No.2, Wuduan, Renmin Street, 121000, Jinzhou, China. yuanyajiang925@sina.com.
First Affiliated Hospital of Liaoning Medical University · CNJinzhou Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aims to investigate the effects of CXCR7-shRNA on TRAIL-mediated apoptosis and suppression of invasive migration and the underlying mechanisms. (1) We constructed CXCR-7-shRNA lentiviral vectors and confirmed their silencing efficiency in MCF-7 cells by RT-PCR analysis. (2) The effects of CXCR7 and/or TRAIL on cell proliferation were examined by MTT assay. (3) Trans well invasion assay was used to examine the effects of CXCR7 silencing and/or TRAIL on MCF-7 cell invasive migration. (4) Expression of Caspase-3, and Caspase-8, and MMP-2 and MMP-9 proteins was examined by Western blot analysis. (1) Viral titers were 2.95 × 10(8) TU/ml, 3.01 × 10(8) TU/ml, 3.26 × 10(8) TU/ml, and 3.08 × 10(8) TU/ml, respectively. (2) CHXR7 shRNAs markedly decreased CXCR7 mRNA expression in MCF-7 cells, among which CXCR7-shRNA-1 showed significantly higher rate of inhibition (P < 0.05). (3) Combination of TRAIL and CXCR7-shRNA-1 resulted in marked suppression of cell proliferation in time-dependent manner (P < 0.05). (4) Cell invasion capacity was inhibited in each experimental group as compared to blank control group at 48 h post treatments (P < 0.05). Among them, combination of TRAIL and CXCR7-shRNA had the highest inhibitory effect (P < 0.05). (5) Western blot analysis indicated that TRAIL alone does not affect CXCR7 expression, but either TRAIL + CXCR7 shRNA or CXCR7 shRNA alone markedly suppressed CXCR7 protein expression. Furthermore, combination of TRAIL and CXCR-7-shRNA significantly increased Caspase-3 and Caspase-8 expression and decreased MMP-2 and MMP-9 expression (P < 0.05). Knock-down of CXCR-7 expression leads to augmented TRAIL-mediated suppression of MCF-7 cell proliferation and invasion.

Indexed as

ApoptosisBreast NeoplasmsCell MovementCell ProliferationFemaleGene Expression Regulation, NeoplasticGenetic VectorsHumansMCF-7 CellsNeoplasm InvasivenessReceptors, CXCRRNA, Small InterferingTNF-Related Apoptosis-Inducing LigandACKR3 protein, humanReceptors, CXCRRNA, Small InterferingTNF-Related Apoptosis-Inducing LigandTNFSF10 protein, humanBreast cancerCXCR7Recombinant lentiviral vectorRNAiTRAIL

Identifiers

PMID25894375
OpenAlexW2091834541

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.