ArticleScientific reports2015
Fast imaging of live organisms with sculpted light sheets.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed.
- Model based optimization for refractive index mismatched light sheet imaging.Optics express · 2024Article
- Full-aperture extended-depth oblique plane microscopy through dynamic remote focusing.Journal of biomedical optics · 2024Article
- Rapid 3D isotropic imaging of whole organ with double-ring light-sheet microscopy and self-learning side-lobe elimination.Biomedical optics express · 2023Article
- Engineering a better light sheet in an axicon-based system using a flattened Gaussian beam of low order.Journal of biophotonics · 2022Article
- Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.Plant physiology · 2021Review
- High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution.Journal of biomedical optics · 2020Article
- Speeded-Up Focus Control of Electrically Tunable Lens by Sparse Optimization.Scientific reports · 2019Article
- Propagating-path uniformly scanned light sheet excitation microscopy for isotropic volumetric imaging of large specimens.Journal of biomedical optics · 2019Article
- Light-sheet microscopy with length-adaptive Bessel beams.Biomedical optics express · 2019Article
- Multiscale light-sheet for rapid imaging of cardiopulmonary system.JCI insight · 2018Review
- Adaptable, illumination patterning light sheet microscopy.Scientific reports · 2018Article
- Homographically generated light sheets for the microscopy of large specimens.Optics letters · 2018Article
- Automatic and adaptive heterogeneous refractive index compensation for light-sheet microscopy.Nature communications · 2017Article
- A guide to light-sheet fluorescence microscopy for multiscale imaging.Nature methods · 2017Review
- Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy.Nature communications · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Light-sheet microscopy is an increasingly popular technique in the life sciences due to its fast 3D imaging capability of fluorescent samples with low photo toxicity compared to confocal methods. In this work we present a new, fast, flexible and simple to implement method to optimize the illumination light-sheet to the requirement at hand. A telescope composed of two electrically tuneable lenses enables us to define thickness and position of the light-sheet independently but accurately within milliseconds, and therefore optimize image quality of the features of interest interactively. We demonstrated the practical benefit of this technique by 1) assembling large field of views from tiled single exposure each with individually optimized illumination settings; 2) sculpting the light-sheet to trace complex sample shapes within single exposures. This technique proved compatible with confocal line scanning detection, further improving image contrast and resolution. Finally, we determined the effect of light-sheet optimization in the context of scattering tissue, devising procedures for balancing image quality, field of view and acquisition speed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.