ArticleJournal of the American Society for Mass Spectrometry2015
High spatial resolution imaging mass spectrometry of human optic nerve lipids and proteins.
Article in Journal of the American Society for Mass Spectrometry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Retinal glial cells in glaucoma and age-related retinal diseases: Inflammatory responses, disease transitions, and translational perspectives.Neural regeneration research · 2026Article
- Spatial biology using single-cell mass spectrometry imaging and integrated microscopy.Nature communications · 2025Article
- Rod-specific downregulation of omega-3 very-long-chain polyunsaturated fatty acid pathway in age-related macular degeneration.Experimental eye research · 2023Article
- Lysolipids are prominent in subretinal drusenoid deposits, a high-risk phenotype in age-related macular degeneration.Frontiers in ophthalmology · 2023Article
- High-Resolution Imaging Mass Spectrometry of Human Donor Eye: Photoreceptors Cells and Basal Laminar Deposit of Age-Related Macular Degeneration.Advances in experimental medicine and biology · 2023Article
- The impact of mass spectrometry application to screen new proteomics biomarkers in Ophthalmology.International ophthalmology · 2021Review
- Mass Spectrometry Imaging of Fibroblasts: Promise and Challenge.Expert review of proteomics · 2021Review
- Lipid Landscape of the Human Retina and Supporting Tissues Revealed by High-Resolution Imaging Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2020Article
- Metabolomic Profiling in Neuromyelitis Optica Spectrum Disorder Biomarker Discovery.Metabolites · 2020Review
- Development and evaluation of matrix application techniques for high throughput mass spectrometry imaging of tissues in the clinic.Clinical mass spectrometry (Del Mar, Calif.) · 2019Article
- Optic Nerve Lipidomics Reveal Impaired Glucosylsphingosine Lipids Pathway in Glaucoma.Investigative ophthalmology & visual science · 2019Article
- Properties, metabolism and roles of sulfogalactosylglycerolipid in male reproduction.Progress in lipid research · 2018Review
- Molecular composition of the human primary visual cortex profiled by multimodal mass spectrometry imaging.Brain structure & function · 2018Article
- Femtosecond laser desorption ionization mass spectrometry imaging and multivariate analysis of lipids in pancreatic tissue.Biointerphases · 2018Article
- Bis(monoacylglycero)phosphate lipids in the retinal pigment epithelium implicate lysosomal/endosomal dysfunction in a model of Stargardt disease and human retinas.Scientific reports · 2017Article
- Advances in MALDI imaging mass spectrometry of proteins in cardiac tissue, including the heart valve.Biochimica et biophysica acta. Proteins and proteomics · 2017Review
- Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia.Scientific reports · 2016Article
- Mass spectrometry in ocular drug research.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The human optic nerve carries signals from the retina to the visual cortex of the brain. Each optic nerve is comprised of approximately one million nerve fibers that are organized into bundles of 800-1200 fibers surrounded by connective tissue and supportive glial cells. Damage to the optic nerve contributes to a number of blinding diseases including: glaucoma, neuromyelitis optica, optic neuritis, and neurofibromatosis; however, the molecular mechanisms of optic nerve damage and death are incompletely understood. Herein we present high spatial resolution MALDI imaging mass spectrometry (IMS) analysis of lipids and proteins to define the molecular anatomy of the human optic nerve. The localization of a number of lipids was observed in discrete anatomical regions corresponding to myelinated and unmyelinated nerve regions as well as to supporting connective tissue, glial cells, and blood vessels. A protein fragment from vimentin, a known intermediate filament marker for astrocytes, was observed surrounding nerved fiber bundles in the lamina cribrosa region. S100B was also found in supporting glial cell regions in the prelaminar region, and the hemoglobin alpha subunit was observed in blood vessel areas. The molecular anatomy of the optic nerve defined by MALDI IMS provides a firm foundation to study biochemical changes in blinding human diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.