ReviewProgress in retinal and eye research2015
Fixation strategies for retinal immunohistochemistry.
Review in Progress in retinal and eye research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed.
- Preparation and evaluation of antisera for immunohistochemical detection of Zika virus in aldehyde-fixed tissuesBiomedica : revista del Instituto Nacional de Salud · 2026Article
- Revisiting the Molecular Architecture of Photoreceptor Ribbon Synapses Using Ultrastructure Expansion Microscopy.Investigative ophthalmology & visual science · 2026Article
- Time-Resolved Oxidative Stress and Apoptosis in Murine Retina under Acute Hypobaric Hypoxia with Parallel Activation of the LIF-JAK-STAT3 Axis.Molecular neurobiology · 2026Article
- Improvement and development of immersion fixatives for enhanced tissue preservation in the whole eyeball specimens.The Journal of veterinary medical science · 2025Article
- Evaluation of Glyoxal fixation for immunohistochemistry of the retina.Scientific reports · 2025Article
- Localization of fluorescent gold nanoparticles throughout the eye after topical administration.Frontiers in medicine · 2025Article
- Article
- The Neuropeptide α-Melanocyte-Stimulating Hormone Prevents Persistent Corneal Edema following Injury.The American journal of pathology · 2024Article
- Unambiguous identification of asymmetric and symmetric synapses using volume electron microscopy.Frontiers in neuroanatomy · 2024Article
- Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography.Biomedical optics express · 2023Article
- Visualizing Protein Localizations in Fixed Cells: Caveats and the Underlying Mechanisms.The journal of physical chemistry. B · 2023Review
- Resolving the molecular architecture of the photoreceptor active zone with 3D-MINFLUX.Science advances · 2022Article
- Immunohistochemical staining of immunoglobulin G in healthy equine, canine, and feline corneas.Veterinary ophthalmology · 2022Article
- Central Role for Adipocyte Na,K-ATPase Oxidant Amplification Loop in the Pathogenesis of Experimental Uremic Cardiomyopathy.Journal of the American Society of Nephrology : JASN · 2020Article
- Canine CD117-Specific Antibodies with Diverse Binding Properties Isolated from a Phage Display Library Using Cell-Based Biopanning.Antibodies (Basel, Switzerland) · 2019Article
- Correlation of spectral domain optical coherence tomography with histology and electron microscopy in the porcine retina.Experimental eye research · 2018Article
- Optimization of fixative solution for retinal morphology: a comparison with Davidson's fixative and other fixation solutions.Japanese journal of ophthalmology · 2018Article
- Non-labeled lensless micro-endoscopic approach for cellular imaging through highly scattering media.Bioscience reports · 2018Article
- Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment.Frontiers in cellular neuroscience · 2016Article
- Functional characterization of LSM12 as a driver in uveal melanoma oncogenesis.Advances in ophthalmology practice and researchArticle
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Immunohistochemical and ex vivo anatomical studies have provided many glimpses of the variety, distribution, and signaling components of vertebrate retinal neurons. The beauty of numerous images published to date, and the qualitative and quantitative information they provide, indicate that these approaches are fundamentally useful. However, obtaining these images entailed tissue handling and exposure to chemical solutions that differ from normal extracellular fluid in composition, temperature, and osmolarity. Because the differences are large enough to alter intercellular and intracellular signaling in neurons, and because retinae are susceptible to crush, shear, and fray, it is natural to wonder if immunohistochemical and anatomical methods disturb or damage the cells they are designed to examine. Tissue fixation is typically incorporated to guard against this damage and is therefore critically important to the quality and significance of the harvested data. Here, we describe mechanisms of fixation; advantages and disadvantages of using formaldehyde and glutaraldehyde as fixatives during immunohistochemistry; and modifications of widely used protocols that have recently been found to improve cell shape preservation and immunostaining patterns, especially in proximal retinal neurons.
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