ArticleExperimental hematology2015
CD201 and CD27 identify hematopoietic stem and progenitor cells across multiple murine strains independently of Kit and Sca-1.
Article in Experimental hematology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functions.Frontiers in immunology · 2025Article
- Integrated transcriptomics of human blood vessels defines a spatially controlled niche for early mesenchymal progenitor cells.Developmental cell · 2024Article
- Hematopoietic stem cells undergo a lymphoid to myeloid switch in early stages of emergency granulopoiesis.The EMBO journal · 2023Article
- Characterizing the role of Phlda3 in the development of acute toxicity and malignant transformation of hematopoietic cells induced by total-body irradiation in mice.Scientific reports · 2023Article
- Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion.Cell stem cell · 2023Article
- Article
- The CD70-CD27 axis in oncology: the new kids on the block.Journal of experimental & clinical cancer research : CR · 2022Review
- Acute and endothelial-specific Robo4 deletion affect hematopoietic stem cell trafficking independent of VCAM1.PloS one · 2021Article
- Inhibiting Glycogen Synthase Kinase-3 Mitigates the Hematopoietic Acute Radiation Syndrome in a Sex- and Strain-dependent Manner in Mice.Health physics · 2020Article
- Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells.Cell reports · 2020Article
- Plinabulin ameliorates neutropenia induced by multiple chemotherapies through a mechanism distinct from G-CSF therapies.Cancer chemotherapy and pharmacology · 2020Article
- CD34 and EPCR coordinately enrich functional murine hematopoietic stem cells under normal and inflammatory conditions.Experimental hematology · 2020Article
- Article
- Review
- The CD11a and Endothelial Protein C Receptor Marker Combination Simplifies and Improves the Purification of Mouse Hematopoietic Stem Cells.Stem cells translational medicine · 2018Article
- Dietary omega-3 and omega-6 polyunsaturated fatty acids modulate hepatic pathology.The Journal of nutritional biochemistry · 2018Article
- CD27 marks murine embryonic hematopoietic stem cells and type II prehematopoietic stem cells.Blood · 2017Article
- SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells.Stem cell reports · 2017Article
- Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model.Journal of immunology research · 2017Article
- NOD Scid Gamma Mice Are Permissive to Allogeneic HSC Transplantation without Prior Conditioning.International journal of molecular sciences · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Identification and isolation of hematopoietic stem cells (HSCs) in mice is most commonly based on the expression of surface molecules Kit and Sca-1 and the absence of markers of mature lineages. However, Sca-1 is absent or weakly expressed in hematopoietic progenitors in many strains, including nonobese diabetic (NOD), BALB/c, C3H, and CBA mice. In addition, both Kit and Sca-1 levels are modulated following bone marrow injury. In these cases, other markers and dye exclusion methods have been employed to identify HSCs, yet there is no antibody-based stain that enables identification of HSCs and early progenitors when Kit and Sca-1 are inadequate. CD201 is a marker that is highly restricted to HSCs and progenitors, and CD27 is expressed at moderate-to-high levels on HSCs. We show here that combining CD201 and CD27 enables highly efficient isolation of long-term HSCs in NOD mice as well as in other strains, including SJL, FVB, AKR, BALB/c, C3H, and CBA. We also find that HSCs appear to maintain expression of CD201 and CD27 after hematopoietic injury when Kit expression is downregulated. These results suggest a widely applicable yet simple alternative for HSC isolation in settings where Kit and Sca-1 expression are insufficient.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.