ArticleScientific reports2015
Mechanism of the antigen-independent cytokinergic SPE-7 IgE activation of human mast cells in vitro.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- LP-003, a novel high-affinity anti-IgE antibody for inadequately controlled seasonal allergic rhinitis: A multicenter, randomized, double-blind, placebo-controlled phase 2 clinical trial.Clinical and translational allergy · 2025Article
- Maternal IgE Influence on Fetal and Infant Health.Immunological reviews · 2025Review
- Article
- Atopy in chronic urticaria: an important yet overlooked issue.Frontiers in immunology · 2024Review
- Mast Cell Desensitization in Allergen Immunotherapy.Frontiers in allergy · 2022Review
- Review
- Total IgE as a Marker for Chronic Spontaneous Urticaria.Allergy, asthma & immunology research · 2021Review
- Novel Approaches in the Inhibition of IgE-Induced Mast Cell Reactivity in Food Allergy.Frontiers in immunology · 2021Review
- IgE Antibodies against Cancer: Efficacy and Safety.Antibodies (Basel, Switzerland) · 2020Review
- Interplay between Affinity and Valency in Effector Cell Degranulation: A Model System with Polcalcin Allergens and Human Patient-Derived IgE Antibodies.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Basophil Activation Test: Old and New Applications in Allergy.Current allergy and asthma reports · 2018Review
- Structure of a patient-derived antibody in complex with allergen reveals simultaneous conventional and superantigen-like recognition.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- IL-3 but not monomeric IgE regulates FcεRI levels and cell survival in primary human basophils.Cell death & disease · 2018Article
- Immunoglobulin E-Mediated Autoimmunity.Frontiers in immunology · 2018Review
- Controlling Mast Cell Activation and Homeostasis: Work Influenced by Bill Paul That Continues Today.Frontiers in immunology · 2018Review
- IgG-Independent Co-aggregation of FcεRI and FcγRIIB Results in LYN- and SHIP1-Dependent Tyrosine Phosphorylation of FcγRIIB in Murine Bone Marrow-Derived Mast Cells.Frontiers in immunology · 2018Article
- IgE Trimers Drive SPE-7 Cytokinergic Activity.Scientific reports · 2017Article
- From IgE to Omalizumab.Journal of immunology (Baltimore, Md. : 1950) · 2016Review
- Fifty years later: Emerging functions of IgE antibodies in host defense, immune regulation, and allergic diseases.The Journal of allergy and clinical immunology · 2016Review
- Differential Lyn-dependence of the SHIP1-deficient mast cell phenotype.Cell communication and signaling : CCS · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Release of pro-inflammatory mediators by mast cells is a key feature of allergic disease. The 'dogma' is that IgE molecules merely sensitise mast cells by binding FcεRI prior to cross-linking by multivalent allergen, receptor aggregation and mast cell activation. However, certain monoclonal IgE antibodies have been shown to elicit mast cell activation in an antigen-independent cytokinergic manner, and DNP-specific murine SPE-7 IgE is the most highly cytokinergic antibody known. We show that both monovalent hapten and recombinant SPE-7 IgE Fab inhibit its cytokinergic activity as measured by mast cell degranulation and TNF-α release. Using SPE-7 IgE, a non-cytokinergic human IgE and a poorly cytokinergic murine IgE, we reveal that interaction of the Fab region of 'free' SPE-7 IgE with the Fab of FcεRI-bound SPE-7 IgE is the basis of its cytokinergic activity. We rule out involvement of IgE Fc, Cε1 and Cλ/κ domains, and propose that 'free' SPE-7 IgE binds to FcεRI-bound SPE-7 IgE by an Fv-Fv interaction. Initial formation of a tri-molecular complex (one 'free' IgE molecule cross-linking two receptor-bound IgE molecules) leads to capture of further 'free' and receptor-bound IgEs to form larger clusters that trigger mast cell activation.
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