ArticleMolecular cell2015
A role for REP sequences in regulating translation.
Article in Molecular cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Article
- Article
- Mechanisms Affecting the Biosynthesis and Incorporation Rate of Selenocysteine.Molecules (Basel, Switzerland) · 2021Review
- Expression of the ISPpu9 transposase of Pseudomonas putida KT2440 is regulated by two small RNAs and the secondary structure of the mRNA 5'-untranslated region.Nucleic acids research · 2021Article
- Article
- TnpAREP and REP sequences dissemination in bacterial genomes: REP recognition determinants.Nucleic acids research · 2021Article
- How sequence populations persist inside bacterial genomes.Genetics · 2021Article
- Mfd regulates RNA polymerase association with hard-to-transcribe regions in vivo, especially those with structured RNAs.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Divergently Transcribed ncRNAs inFrontiers in molecular biosciences · 2021Article
- Ribosome collisions alter frameshifting at translational reprogramming motifs in bacterial mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors.PLoS genetics · 2018Article
- Single-strand DNA processing: phylogenomics and sequence diversity of a superfamily of potential prokaryotic HuH endonucleases.BMC genomics · 2018Article
- Conserved small mRNA with an unique, extended Shine-Dalgarno sequence.RNA biology · 2017Article
- Identification and Characterization of Domesticated Bacterial Transposases.Genome biology and evolution · 2017Article
- Discovering Complete Quasispecies in Bacterial Genomes.Genetics · 2017Article
- Global analysis of protein lysine succinylation profiles in common wheat.BMC genomics · 2017Article
- Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use.Nucleic acids research · 2016Review
- REP sequences: Mediators of the environmental stress response?RNA biology · 2016Article
- Small Open Reading Frames, Non-Coding RNAs and Repetitive Elements in Bradyrhizobium japonicum USDA 110.PloS one · 2016Article
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3 authors.
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Abstract
Repetitive extragenic palindromic (REP) sequences are highly structured elements found downstream of ∼500 genes in Escherichia coli that result in extensive stem-loop structures in their mRNAs. However, their physiological role has remained elusive. Here, we show that REP sequences can downregulate translation, but only if they are within 15 nt of a termination codon; a spacing of 16 nt has no effect, suggesting that the REP element acts to stall ribosome movement. Ribosome stalling leads to cleavage of the mRNA and induction of the trans-translation process. Using nrdAB as a model, we find that its regulation can be partially reversed by overexpression of RNA helicases and can be fully overcome upon UV stress, emphasizing the importance of this regulatory process. Since 50% of REP-associated genes have these elements within the critical 15 nt, these findings identify a regulatory mechanism with the potential to affect translation from a large number of genes.
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