ArticleScientific reports2015
Spatiotemporal distribution of different extracellular polymeric substances and filamentation mediate Xylella fastidiosa adhesion and biofilm formation.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 104 citations in OpenAlex.
- Molecular imaging of bacterial biofilms-a systematic review.Critical reviews in microbiology · 2024Pooled it
- Genome-guided prebiotic fermentation generates anti-virulence metabolites againstFrontiers in microbiology · 2026Article
- Biofilm as a Key Element in the Bacterial Pathogenesis of Forest Trees: A Review of Mechanisms and Ecological Implications.Microorganisms · 2025Review
- Reply to Almeida, "Unreliable evidence to support a vector regulation hypothesis forApplied and environmental microbiology · 2025Article
- Efficiency, Microbial Communities, and Nitrogen Metabolism in Denitrification Biological Filter: Insights into Varied Pore Ceramsite Media.Microorganisms · 2025Article
- The vector regulation hypothesis: dynamic competition between pathogen and vector behaviors constrainsApplied and environmental microbiology · 2024Article
- Article
- Biofilm-Forming Ability of Phytopathogenic Bacteria: A Review of its Involvement in Plant Stress.Plants (Basel, Switzerland) · 2023Review
- Deletion ofInternational journal of molecular sciences · 2023Article
- Article
- Controlled spatial organization of bacterial growth reveals key role of cell filamentation preceding Xylella fastidiosa biofilm formation.NPJ biofilms and microbiomes · 2021Article
- Computational characterizations of GDP-mannose 4,6-dehydratase (NoeL) Rhizobial proteins.Current genetics · 2021Article
- Functionalized microchannels as xylem-mimicking environment: Quantifying X. fastidiosa cell adhesion.Biophysical journal · 2021Article
- Review
- Antimicrobial Peptides With Antibiofilm Activity AgainstFrontiers in microbiology · 2021Article
- Phenotypic Characterization and Transformation Attempts Reveal Peculiar Traits ofMicroorganisms · 2020Article
- Competitive and/or Cooperative Interactions ofFrontiers in microbiology · 2020Article
- Non-Lethal Effects ofMicroorganisms · 2019Article
- Testing Anti-Biofilm Polymeric Surfaces: Where to Start?International journal of molecular sciences · 2019Review
- Cell-length heterogeneity: a population-level solution to growth/virulence trade-offs in the plant pathogen Dickeya dadantii.PLoS pathogens · 2019Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microorganism pathogenicity strongly relies on the generation of multicellular assemblies, called biofilms. Understanding their organization can unveil vulnerabilities leading to potential treatments; spatially and temporally-resolved comprehensive experimental characterization can provide new details of biofilm formation, and possibly new targets for disease control. Here, biofilm formation of economically important phytopathogen Xylella fastidiosa was analyzed at single-cell resolution using nanometer-resolution spectro-microscopy techniques, addressing the role of different types of extracellular polymeric substances (EPS) at each stage of the entire bacterial life cycle. Single cell adhesion is caused by unspecific electrostatic interactions through proteins at the cell polar region, where EPS accumulation is required for more firmly-attached, irreversibly adhered cells. Subsequently, bacteria form clusters, which are embedded in secreted loosely-bound EPS, and bridged by up to ten-fold elongated cells that form the biofilm framework. During biofilm maturation, soluble EPS forms a filamentous matrix that facilitates cell adhesion and provides mechanical support, while the biofilm keeps anchored by few cells. This floating architecture maximizes nutrient distribution while allowing detachment upon larger shear stresses; it thus complies with biological requirements of the bacteria life cycle. Using new approaches, our findings provide insights regarding different aspects of the adhesion process of X. fastidiosa and biofilm formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.