ArticleBiomaterials2015
Enhanced immunostimulatory effects of DNA-encapsulated peptide hydrogels.
Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 68 citations in OpenAlex.
- Exploring the temperature dependence of β-hairpin peptide self-assembly.Faraday discussions · 2025Article
- A self-assembled protein β-helix as a self-contained biofunctional motif.Nature communications · 2025Article
- DNA-based hydrogels for bone regeneration: A promising tool for bone organoids.Materials today. Bio · 2025Review
- Fabrication of levofloxacin-loaded porcine acellular dermal matrix hydrogel and functional assessment in urinary tract infection.Journal of nanobiotechnology · 2024Article
- Review
- The Effects of Charged Amino Acid Side-Chain Length on Diagonal Cross-Strand Interactions between Carboxylate- and Ammonium-Containing Residues in a β-Hairpin.Molecules (Basel, Switzerland) · 2022Article
- Peptide hydrogels for affinity-controlled release of therapeutic cargo: Current and potential strategies.Journal of peptide science : an official publication of the European Peptide Society · 2022Review
- Defining the Landscape of the Pauling-Corey Rippled Sheet: An Orphaned Motif Finding New Homes.Accounts of chemical research · 2021Review
- Drug-Mimicking Nanofibrous Peptide Hydrogel for Inhibition of Inducible Nitric Oxide Synthase.ACS biomaterials science & engineering · 2019Article
- Design of a Peptide-Based Electronegative Hydrogel for the Direct Encapsulation, 3D Culturing, in Vivo Syringe-Based Delivery, and Long-Term Tissue Engraftment of Cells.ACS applied materials & interfaces · 2019Article
- Preparation and applications of peptide-based injectable hydrogels.RSC advances · 2019Review
- Peptide Tectonics: Encoded Structural Complementarity Dictates Programmable Self-Assembly.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Review
- Design of a Multicompartment Hydrogel that Facilitates Time-Resolved Delivery of Combination Therapy and Synergized Killing of Glioblastoma.Angewandte Chemie (International ed. in English) · 2018Article
- Encapsulation of Biological Agents in Hydrogels for Therapeutic Applications.Gels (Basel, Switzerland) · 2018Review
- Macromolecule-Network Electrostatics Controlling Delivery of the Biotherapeutic Cell Modulator TIMP-2.Biomacromolecules · 2018Article
- Biodegradable Poly(Amino Ester) with Aromatic Backbone as Efficient Nonviral Gene Delivery Vectors.Molecules (Basel, Switzerland) · 2017Article
- β-hairpin peptide hydrogels for package delivery.Advanced drug delivery reviews · 2017Review
- Peptide-Based Supramolecular Hydrogels for Delivery of Biologics.Bioengineering & translational medicine · 2016Article
- The Enzyme-instructed assembly of the core of yeast prion Sup35 to form supramolecular hydrogels.Journal of materials chemistry. B · 2016Article
- Biosynthesis and Characterization of Cross-Linked Fmoc Peptide-Based Hydrogels for Drug Delivery Applications.Gels (Basel, Switzerland) · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
DNA that encodes tumor-specific antigens represents potential immunostimulatory agents. However, rapid enzymatic degradation and fragmentation of DNA during administration can result in limited vector expression and, consequently, poor efficacy. These challenges have necessitated the use of novel strategies for DNA delivery. Herein, we study the ability of cationic self-assembling peptide hydrogels to encapsulate plasmid DNA, and enhance its immunostimulatory potential in vivo. The effect of network charge on the gel's ability to retain the DNA was assessed employing three gel-forming peptides that vary systematically in formal charge. The peptide HLT2, having a formal charge of +5 at neutral pH, was optimal in encapsulating microgram quantities of DNA with little effect on its rheological properties, allowing its effective syringe delivery in vivo. The plasmid, DNA(TA), encapsulated within these gels encodes for a melanoma-specific gp100 antigen fused to the alarmin protein adjuvant HMGN1. Implantation of DNA(TA)-loaded HLT2 gels into mice resulted in an acute inflammatory response with the presence of polymorphonuclear cells, which was followed by infiltrating macrophages. These cellular infiltrates aid in the processing of encapsulated DNA, promoting increased lymphoproliferation and producing an enhanced immune response mediated by CD4+/IFNγ+ expressing Th1 cells, and complemented by the formation of gp100-specific antibodies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.