ArticleJournal of orthopaedic surgery and research2015
A preliminary study of osteochondral regeneration using a scaffold-free three-dimensional construct of porcine adipose tissue-derived mesenchymal stem cells.
Article in Journal of orthopaedic surgery and research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 3 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 3 syntheses or guidelines pooled it, 79 citations in OpenAlex.
- Cell therapies for chondral defects of the talus: a systematic review.Journal of orthopaedic surgery and research · 2022Pooled it
- Pooled it
- The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review.Journal of orthopaedic surgery and research · 2017Pooled it
- Lateral Ramus Cortical Bone Plate in Alveolar Cleft Osteoplasty with Concomitant Use of Buccal Fat Pad Derived Cells and Autogenous Bone: Phase I Clinical Trial.BioMed research international · 2017Trial
- Tracking Spatiotemporal Extracellular Matrix Evolution and Tissue Fusion in 3D Microtissues via Click Chemistry-Based Metabolic Labelling.Advanced healthcare materials · 2026Article
- Response to the letter to the editor regarding "Bio-3D printing of scaffold-free ADSC-derived cartilage constructs comparable to natural cartilage in vitro".Journal of orthopaedic surgery and research · 2026Article
- Enhanced Bone Regeneration by Scaffold-Free Three-Dimensional Constructs of Human Dental Pulp Stem Cells in a Rat Mandibular Defect Model.International journal of molecular sciences · 2026Article
- Xenograft of bio-3D printed scaffold-free cartilage constructs derived from human iPSCs to regenerate articular cartilage in immunodeficient pigs.Regenerative therapy · 2025Article
- Scaffold-free 3D culture systems for stem cell-based tissue regeneration.APL bioengineering · 2024Review
- Bone Regeneration of Rat Critical-Sized Calvarial Defects by the Combination of Photobiomodulation and Adipose-Derived Mesenchymal Stem Cells.Journal of lasers in medical sciences · 2024Article
- Articular Cartilage Regeneration through Bioassembling Spherical Micro-Cartilage Building Blocks.Cells · 2022Review
- Review
- Implication of Mesenchymal Stem Cells and Their Derivates for Osteochondral Regeneration.International journal of molecular sciences · 2022Review
- Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses.BMC veterinary research · 2022Article
- Fracture Healing Research-Shift towards In Vitro Modeling?Biomedicines · 2021Review
- Comparison of Genetically Engineered Immunodeficient Animal Models for Nonclinical Testing of Stem Cell Therapies.Pharmaceutics · 2021Review
- Mesangiogenic Progenitor Cells Are Tissue Specific and Cannot Be Isolated From Adipose Tissue or Umbilical Cord Blood.Frontiers in cell and developmental biology · 2021Article
- Osteochondral regeneration using scaffold-free constructs of adipose tissue-derived mesenchymal stem cells made by a bio three-dimensional printer with a needle-array in rabbits.Regenerative therapy · 2020Article
- Osteochondral Regeneration Using Adipose Tissue-Derived Mesenchymal Stem Cells.International journal of molecular sciences · 2020Review
- Computational fluid dynamic analysis of bioprinted self-supporting perfused tissue models.Biotechnology and bioengineering · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOsteoarthritis (OA) is a major joint disease in humans and many other animals. Consequently, medical countermeasures for OA have been investigated diligently. This study was designed to examine the regeneration of articular cartilage and subchondral bone using three-dimensional (3D) constructs of adipose tissue-derived mesenchymal stem cells (AT-MSCs).
methodsAT-MSCs were isolated and expanded until required for genetical and immunological analysis and construct creation. A construct consisting of about 760 spheroids that each contained 5.0 × 10(4) autologous AT-MSCs was implanted into an osteochondral defect (diameter: 4 mm; depth: 6 mm) created in the femoral trochlear groove of two adult microminipigs. After implantation, the defects were monitored by computed tomography every month for 6 months in animal no. 1 and 12 months in animal no. 2.
resultsAT-MSCs were confirmed to express the premature genes and to be positive for CD90 and CD105 and negative for CD34 and CD45. Under specific nutrient conditions, the AT-MSCs differentiated into osteogenic, chondrogenic, and adipogenic lineages, as evidenced by the expressions of related marker genes and the production of appropriate matrix molecules. A radiopaque area emerged from the boundary between the bone and the implant and increased more steadily upward and inward for the implants in both animal no. 1 and animal no. 2. The histopathology of the implants after 6 months revealed active endochondral ossification underneath the plump fibrocartilage in animal no. 1. The histopathology after 12 months in animal no. 2 showed not only that the diminishing fibrocartilage was as thick as the surrounding normal cartilage but also that massive subchondral bone was present.
conclusionsThe present results suggest that implantation of a scaffold-free 3D construct of AT-MSCs into an osteochondral defect may induce regeneration of the original structure of the cartilage and subchondral bone over the course of 1 year, although more experimental cases are needed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.