ArticleMalaria journal2015
Splenic architecture disruption and parasite-induced splenocyte activation and anergy in Plasmodium falciparum-infected Saimiri sciureus monkeys.
Article in Malaria journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
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- Severe malaria enforces short-lived effector cell differentiation but does not prevent effective secondary responses by memory CD8 T cells.PLoS pathogens · 2025Article
- IL-15 Complex-Induced IL-10 Enhances Plasmodium-specific CD4+ T Follicular Helper Differentiation and Antibody Production.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- A cluster of broadly neutralizing IgG against BK polyomavirus in a repertoire dominated by IgM.Life science alliance · 2023Article
- Eryptosis as a New Insight in Malaria Pathogenesis.Frontiers in immunology · 2022Review
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- Plasmodium falciparum-specific IgM B cells dominate in children, expand with malaria, and produce functional IgM.The Journal of experimental medicine · 2021Article
- T-follicular helper cells in malaria infection and roles in antibody induction.Oxford open immunology · 2021Review
- B cells are sufficient to prime the dominant CD4+ Tfh response to Plasmodium infection.The Journal of experimental medicine · 2020Article
- Using two phases of the CD4 T cell response to blood-stage murine malaria to understand regulation of systemic immunity and placental pathology in Plasmodium falciparum infection.Immunological reviews · 2020Review
- Plasmodium co-infection protects against chikungunya virus-induced pathologies.Nature communications · 2018Article
- cGAS-mediated control of blood-stage malaria promotes Plasmodium-specific germinal center responses.JCI insight · 2018Article
- Histological Disorganization of Spleen Compartments and Severe Visceral Leishmaniasis.Frontiers in cellular and infection microbiology · 2018Review
- IL-17 Promotes Differentiation of Splenic LSKJournal of immunology (Baltimore, Md. : 1950) · 2017Article
- Avidity of anti-malarial antibodies inversely related to transmission intensity at three sites in Uganda.Malaria journal · 2017Article
- IncreasedFrontiers in cellular and infection microbiology · 2017Article
- Malaria Parasites: The Great Escape.Frontiers in immunology · 2016Review
- Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research.Malaria journal · 2015Article
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe understanding of the mechanisms of immunity in malaria is crucial for the rational development of interventions such as vaccines. During blood stage infection, the spleen is considered to play critical roles in both immunity and immunopathology of Plasmodium falciparum infections.
methodsSaimiri sciureus monkeys were inoculated with blood stages of P. falciparum (FUP strain) and spleens removed during acute disease (days 7 and 13 of infection) and during convalescence (15 days after start of chloroquine treatment). Cytokine (IFNγ, TNFα, IL2, IL6, IL10, and IL12) responses of splenocytes stimulated with P. falciparum-parasitized red blood cells were assessed by real-time PCR using specific Saimiri primers, and histological changes were evaluated using haematoxylin-eosin and Giemsa-stained slides.
resultsEarly during infection (day 7, 1-2% parasitaemia), spleens showed disruption of germinal centre architecture with heavy B-cell activation (centroblasts), and splenocytes showed increased expression of IFNγ, IL6 and IL12 upon in vitro stimuli by P. falciparum-parasitized red blood cells (pRBC). Conversely, 15 days after treatment of blood stage infection with chloroquine, splenocytes showed spontaneous in vitro expression of TNFα, IL2, IL6, IL10, and IL12, but not IFNγ, and stimulation with P. falciparum pRBC blocked the expression of all these cytokines. During the acute phase of infection, splenic disarray with disorganized germinal centres was observed. During convalescence, spleens of the chloroquine-treated animals showed white pulp hyperplasia with extensive lymphocyte activation and persistency of heavily haemozoin-laden macrophages throughout the red pulp.
conclusionsInability to eliminate haemozoin is likely involved in the persistent lymphocyte activation and in the anergic responses of Saimiri splenocytes to P. falciparum pRBC, with important negative impact in immune responses and implications for the design of malaria vaccine.
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