ArticleStem cell research & therapy2015
ARP2/3 complex is required for directional migration of neural stem cell-derived oligodendrocyte precursors in electric fields.
Article in Stem cell research & therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Application of oscillating field stimulation in the treatment of spinal cord injury: a systematic review and meta-analysis of preclinical studies.Journal of neuroengineering and rehabilitation · 2025Pooled it
- Cochlear-bioelectrode: Auditory response to cochlear implant can be improved by stem cell delivery into inner ear in a pig model.Stem cell research & therapy · 2025Article
- Molecular mechanisms of hotspot variants in cytoskeletal β-actin associated with Baraitser-Winter syndrome.The FEBS journal · 2025Article
- The Arp2/3 complex enhances cell migration on elastic substrates.Molecular biology of the cell · 2023Article
- Collagen Matrices Mediate Glioma Cell Migration Induced by an Electrical Signal.Gels (Basel, Switzerland) · 2022Article
- Protocadherin 15 suppresses oligodendrocyte progenitor cell proliferation and promotes motility through distinct signalling pathways.Communications biology · 2022Article
- Neurogenesis potential of oligodendrocyte precursor cells from oligospheres and injured spinal cord.Frontiers in cellular neuroscience · 2022Article
- Entacapone Treatment Modulates Hippocampal Proteins Related to Synaptic Vehicle Trafficking.Cells · 2020Article
- Genetic analysis of the molecular regulation of electric fields-guided glia migration.Scientific reports · 2020Article
- Review
- Weak direct current (DC) electric fields as a therapy for spinal cord injuries: review and advancement of the oscillating field stimulator (OFS).Neurosurgical review · 2019Review
- Temporal and partial inhibition of GLI1 in neural stem cells (NSCs) results in the early maturation of NSC derived oligodendrocytes in vitro.Stem cell research & therapy · 2019Article
- Live-cell Migration Assays to Study Motility of Neural andGlial (Oligodendrocyte) Progenitor Cells.Bio-protocol · 2019Article
- Low intensity transcranial electric stimulation: Safety, ethical, legal regulatory and application guidelines.Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology · 2017Review
- Subcellular electrical stimulation of neurons enhances the myelination of axons by oligodendrocytes.PloS one · 2017Article
- Electric Signals Regulate the Directional Migration of Oligodendrocyte Progenitor Cells (OPCs) via β1 Integrin.International journal of molecular sciences · 2016Article
- Function and regulation of the Arp2/3 complex during cell migration in diverse environments.Current opinion in cell biology · 2016Review
- Crucial roles of the Arp2/3 complex during mammalian corticogenesis.Development (Cambridge, England) · 2016Article
- The Role of Direct Current Electric Field-Guided Stem Cell Migration in Neural Regeneration.Stem cell reviews and reports · 2016Review
- Dynamic behaviors of astrocytes in chemically modified fibrin and collagen hydrogels.Integrative biology : quantitative biosciences from nano to macro · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
introductionThe loss of oligodendrocytes in a lesion of the central nervous system causes demyelination and therefore impairs axon function and survival. Transplantation of neural stem cell-derived oligodendrocyte precursor cells (NSC-OPCs) results in increased oligodendrocyte formation and enhanced remyelination. The directional migration of grafted cells to the target can promote the establishment of functional reconnection and myelination in the process of neural regeneration. Endogenous electric fields (EFs) that were detected in the development of the central nervous system can regulate cell migration.
methodsNSCs were isolated from the brains of ARPC2+/+ and ARPC2-/- mouse embryo and differentiated into OPCs. After differentiation, the cultured oligospheres were stimulated with EFs (50, 100, or 200 mV/mm). The migration of OPCs from oligospheres was recorded using time-lapse microscopy. The cell migration directedness and speed were analyzed and quantified.
resultsIn this study, we found that NSC-OPCs migrated toward the cathode pole in EFs. The directedness and displacement of cathodal migration increased significantly when the EF strength increased from 50 to 200 mV/mm. However, the EF did not significantly change the cell migration speed. We also showed that the migration speed of ARPC2-/- OPCs, deficient in the actin-related proteins 2 and 3 (ARP2/3) complex, was significantly lower than that of wild type of OPCs. ARPC2-/- OPCs migrated randomly in EFs.
conclusionsThe migration direction of NSC-OPCs can be controlled by EFs. The function of the ARP complex is required for the cathodal migration of NSC-OPCs in EFs. EF-guided cell migration is an effective model to understanding the intracellular signaling pathway in the regulation of cell migration directness and motility.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.