ArticleJournal of translational medicine2015
Characterization of a novel endothelial biosensor assay reveals increased cumulative serum inflammatory potential in stabilized coronary artery disease patients.
Article in Journal of translational medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Serum ICAM-1 as a Predictor of Prognosis in Patients with Acute Ischemic Stroke.BioMed research international · 2021Trial
- Air Pollution Exposure Induces Vascular Endothelial Cell Dysfunction via Elevated Circulating F2: Multiomics Insights from anEnvironment & health (Washington, D.C.) · 2026Article
- Circulatory metabolites trigger ex vivo arterial endothelial cell dysfunction in population chronically exposed to diesel exhaust.Particle and fibre toxicology · 2022Article
- VCAM-1 Is Upregulated in Uranium Miners Compared to Other Miners.Life (Basel, Switzerland) · 2021Article
- Pulmonary delivery of the broad-spectrum matrix metalloproteinase inhibitor marimastat diminishes multiwalled carbon nanotube-induced circulating bioactivity without reducing pulmonary inflammation.Particle and fibre toxicology · 2021Article
- Mine-site derived particulate matter exposure exacerbates neurological and pulmonary inflammatory outcomes in an autoimmune mouse model.Journal of toxicology and environmental health. Part A · 2021Article
- Occupational exposure to carbon black nanoparticles increases inflammatory vascular disease risk: an implication of an ex vivo biosensor assay.Particle and fibre toxicology · 2020Article
- Serum-borne factors alter cerebrovascular endothelial microRNA expression following particulate matter exposure near an abandoned uranium mine on the Navajo Nation.Particle and fibre toxicology · 2020Article
- Toxic Effects of Particulate Matter Derived from Dust Samples Near the Dzhidinski Ore Processing Mill, Eastern Siberia, Russia.Cardiovascular toxicology · 2019Article
- Acute exposure to e-cigarettes causes inflammation and pulmonary endothelial oxidative stress in nonsmoking, healthy young subjects.American journal of physiology. Lung cellular and molecular physiology · 2019Article
- Residential proximity to abandoned uranium mines and serum inflammatory potential in chronically exposed Navajo communities.Journal of exposure science & environmental epidemiology · 2017Article
- Serum from obstructive sleep apnea patients induces inflammatory responses in coronary artery endothelial cells.Atherosclerosis · 2016Article
- Inflammatory and Vasoactive Effects of Serum Following Inhalation of Varied Complex Mixtures.Cardiovascular toxicology · 2016Article
- Potential Diagnostic and Prognostic Biomarkers of Epigenetic Drift within the Cardiovascular Compartment.BioMed research international · 2016Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundVascular disease is promoted by systemic inflammation that can arise from sites distal to the affected vessels. We sought to characterize the net inflammatory potential of serum from patients with coronary artery disease (CAD) using cultured endothelial cells as a cumulative biosensor. METHODS AND
resultsSerum samples from CAD patients (N = 45) and healthy control subjects (N = 48) were incubated with primary human coronary artery endothelial cells at a 1:10 dilution for 4 h, followed by isolation of the cellular RNA. Alteration of inflammation-responsive elements (adhesion molecules and cytokines) was assessed by gene expression. Specific indicators included intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and interleukin-8 (IL-8). Additionally, the cytokine levels in serum samples from all subjects were quantified. Serum from CAD subjects induced greater endothelial ICAM-1, VCAM-1, and IL-8 expression compared to healthy control serum (p < 0.001 for each analysis). The three indicators of inflammatory potential (ICAM-1, VCAM-1, and IL-8 mRNA) trended independently of each other and also of serum inflammatory biomarkers. IL-8 expression correlated negatively with serum HDL levels but positively correlated with VLDL, plasminogen activator inhibitor-1 and C-reactive protein. Interestingly, serum levels of cytokines in CAD patients were not statistically different from healthy control subjects. A year of follow-up in a sub-group of CAD subjects revealed relatively stable measures.
conclusionsAs yet unidentified circulating factors in the serum of CAD patients appear to activate endothelial cells, leading to upregulation of adhesion molecules and chemokines. This cumulative assay performed well in terms of discriminating patients with CAD compared to healthy subjects, with greater range and specificity than specific inflammatory markers.
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