ArticleVirology journal2015
RNA interference-based resistance in transgenic tomato plants against Tomato yellow leaf curl virus-Oman (TYLCV-OM) and its associated betasatellite.
Article in Virology journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 68 citations in OpenAlex.
- amiR414e-mediated silencing confers resistance to Cotton leaf curl Multan virus in cotton.Molecular biology reports · 2026Article
- Enhanced Soybean Immunity to the Soybean Mosaic Virus Through RNA Interference Targeting thePlants (Basel, Switzerland) · 2026Article
- Profiling of extracellular vesicle-associated microRNAs reveals a regulated response to potato virus Y infection in tomato.Frontiers in genetics · 2026Article
- A model-guided alternating high-temperature thermotherapy achieves complete eradication of sugarcane mosaic virus and preserves physiological integrity inFrontiers in plant science · 2026Article
- Hairpin-RNA spray confers resistance to mungbean yellow mosaic India virus in mungbean.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- RNA silencing: the future potential strategy for engineering virus resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Identification of pathogenicity determinants in ToLCNDV and their RNAi-based knockdown for disease management inFrontiers in microbiology · 2024Article
- Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus.Frontiers in plant science · 2023Article
- Current status, breeding strategies and future prospects for managing chilli leaf curl virus disease and associated begomoviruses in Chilli (Frontiers in plant science · 2023Review
- Comparative evaluation of resistance to potato virus Y (PVY) in three different RNAi-based transgenic potato plants.Transgenic research · 2022Article
- Natural resistance of tomato plants to Tomato yellow leaf curl virus.Frontiers in plant science · 2022Review
- Fungal F8-Culture Filtrate Induces Tomato Resistance against Tomato Yellow Leaf Curl Thailand Virus.Viruses · 2021Article
- Engineering tolerance to CLCuD in transgenic Gossypium hirsutum cv. HS6 expressing Cotton leaf curl Multan virus-C4 intron hairpin.Scientific reports · 2021Article
- The Global Dimension of Tomato Yellow Leaf Curl Disease: Current Status and Breeding Perspectives.Microorganisms · 2021Review
- Current Status and Potential of RNA Interference for the Management of Tomato Spotted Wilt Virus and Thrips Vectors.Pathogens (Basel, Switzerland) · 2021Review
- CRISPR/Cas9-Mediated Generation of Pathogen-Resistant Tomato againstInternational journal of molecular sciences · 2021Article
- siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants.Scientific reports · 2020Article
- RNAi-Mediated Resistance Against Viruses in Perennial Fruit Plants.Plants (Basel, Switzerland) · 2019Review
- Article
- RNAi-Mediated Simultaneous Resistance Against Three RNA Viruses in Potato.Molecular biotechnology · 2017Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTomato yellow leaf curl virus (TYLCV), a monopartite begomovirus (family Geminiviridae) is responsible for heavy yield losses for tomato production around the globe. In Oman at least five distinct begomoviruses cause disease in tomato, including TYLCV. Unusually, TYLCV infections in Oman are sometimes associated with a betasatellite (Tomato leaf curl betasatellite [ToLCB]; a symptom modulating satellite). RNA interference (RNAi) can be used to develop resistance against begomoviruses at either the transcriptional or post-transcriptional levels.
resultsA hairpin RNAi (hpRNAi) construct to express double-stranded RNA homologous to sequences of the intergenic region, coat protein gene, V2 gene and replication-associated gene of Tomato yellow leaf curl virus-Oman (TYLCV-OM) was produced. Initially, transient expression of the hpRNAi construct at the site of virus inoculation was shown to reduce the number of plants developing symptoms when inoculated with either TYLCV-OM or TYLCV-OM with ToLCB-OM to Nicotiana benthamiana or tomato. Solanum lycopersicum L. cv. Pusa Ruby was transformed with the hpRNAi construct and nine confirmed transgenic lines were obtained and challenged with TYLCV-OM and ToLCB-OM by Agrobacterium-mediated inoculation. For all but one line, for which all plants remained symptomless, inoculation with TYLCV-OM led to a proportion (≤25%) of tomato plants developing symptoms of infection. For inoculation with TYLCV-OM and ToLCB-OM all lines showed a proportion of plants (≤45%) symptomatic. However, for all infected transgenic plants the symptoms were milder and virus titre in plants was lower than in infected non-transgenic tomato plants.
conclusionsThese results show that RNAi can be used to develop resistance against geminiviruses in tomato. The resistance in this case is not immunity but does reduce the severity of infections and virus titer. Also, the betasatellite may compromise resistance, increasing the proportion of plants which ultimately show symptoms.
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