ArticleMicrobial cell factories2015
Metabolic and genetic factors affecting the productivity of pyrimidine nucleoside in Bacillus subtilis.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 44 citations in OpenAlex.
- Advances in metabolic engineering of Bacillus subtilis towards high-efficiency cytidine biosynthesis.World journal of microbiology & biotechnology · 2026Review
- Pyrimidine sufficiency is required for Sae two-component system signaling inJournal of bacteriology · 2025Article
- Promoting cytidine biosynthesis by modulating pyrimidine metabolism and carbon metabolic regulatory networks in Bacillus subtilis.Microbial cell factories · 2025Article
- Recent advances in microbial synthesis of free heme.Applied microbiology and biotechnology · 2024Review
- The characteristics of autolysins associated with cell separation inJournal of bacteriology · 2024Article
- Research advances on the consolidated bioprocessing of lignocellulosic biomass.Engineering microbiology · 2024Review
- Improving Surfactin Production inMicroorganisms · 2024Article
- A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century.Microbial biotechnology · 2023Review
- NupR Responding to Multiple Signals Is a Nucleoside Permease Regulator in Bacillus thuringiensis BMB171.Microbiology spectrum · 2022Article
- Microbial Succession and Interactions During the Manufacture of Fu Brick Tea.Frontiers in microbiology · 2022Article
- Overexpression of uracil permease and nucleoside transporter from Bacillus amyloliquefaciens improves cytidine production in Escherichia coli.Biotechnology letters · 2021Article
- Article
- Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine.Microbial cell factories · 2016Article
- Deoxycytidine production by a metabolically engineered Escherichia coli strain.Microbial cell factories · 2015Article
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4 authors at 1 institution in 1 country.
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Abstract
backgroundCytidine and uridine are produced commercially by Bacillus subtilis. The production strains of cytidine and uridine were both derivatives from mutagenesis. However, the exact metabolic and genetic factors affecting the productivity remain unknown. Genetic engineering may be a promising approach to identify and confirm these factors.
resultsWith the deletion of the cdd and hom genes, and the deregulation of the pyr operon in Bacillus subtilis168, the engineered strain produced 200.9 mg/L cytidine, 14.9 mg/L uridine and 960.1 mg/L uracil. Then, the overexpressed prs gene led to a dramatic increase of uridine by 25.9 times along with a modest increase of cytidine. Furthermore, the overexpressed pyrG gene improved the production of cytidine, uridine and uracil by 259.5%, 11.2% and 68.8%, respectively. Moreover, the overexpression of the pyrH gene increasesd the yield of cytidine by 40%, along with a modest augments of uridine and uracil. Lastly, the deletion of the nupC-pdp gene resulted in a doubled production of uridine up to 1684.6 mg/L, a 14.4% increase of cytidine to 1423 mg/L, and a 99% decrease of uracil to only 14.2 mg/L.
conclusionsThe deregulation of the pyr operon and the overexpression of the prs, pyrG and pyrH genes all contribute to the accumulation of pyrimidine nucleoside compounds in the medium. Among these factors, the overexpression of the pyrG and pyrH genes can particularly facilitate the production of cytidine. Meanwhile, the deletion of the nupC-pdp gene can obviously reduce the production of uracil and simultaneously improve the production of uridine.
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