ArticleParasites & vectors2015
Mitochondrial and nuclear ribosomal DNA dataset supports that Paramphistomum leydeni (Trematoda: Digenea) is a distinct rumen fluke species.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Complete mitochondrial genome and phylogenetic implications ofInternational journal for parasitology. Parasites and wildlife · 2025Article
- First Molecular Identification ofVeterinary sciences · 2023Article
- Identification and prevalence of fluke infection in yak and Tibetan sheep around Qinghai Lake, China.Parasitology research · 2023Article
- Characterization of the complete mitochondrial genome of Plagiorchis multiglandularis (Digenea, Plagiorchiidae): Comparison with the members of Xiphidiatan species and phylogenetic implications.Parasitology research · 2023Article
- The complete mitochondrial genome ofParasitology · 2023Article
- Characterization of the complete mitochondrial genomes ofInternational journal for parasitology. Parasites and wildlife · 2022Article
- The mitogenome of Ophidascaris wangi isolated from snakes in China.Parasitology research · 2021Article
- A Universal Approach to Molecular Identification of Rumen Fluke Species Across Hosts, Continents, and Sample Types.Frontiers in veterinary science · 2020Article
- The complete mitochondrial genome ofPeerJ · 2019Article
- Fasciolopsis buski (Digenea: Fasciolidae) from China and India may represent distinct taxa based on mitochondrial and nuclear ribosomal DNA sequences.Parasites & vectors · 2017Article
- The complete mitochondrial genomes of Gnathostoma doloresi from China and Japan.Parasitology research · 2016Article
- Complete mitochondrial genome of the giant liver fluke Fascioloides magna (Digenea: Fasciolidae) and its comparison with selected trematodes.Parasites & vectors · 2016Article
- Rumen fluke in Irish sheep: prevalence, risk factors and molecular identification of two paramphistome species.BMC veterinary research · 2016Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRumen flukes parasitize the rumen and reticulum of ruminants, causing paramphistomiasis. Over the years, there has been considerable debate as to whether Paramphistomum leydeni and Paramphistomum cervi are the same or distant species.
methodsIn the present study, the complete mitochondrial (mt) genome of P. leydeni was amplified using PCR-based sequencing and compared with that of P. cervi. The second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA (rDNA) of P. leydeni specimens (n = 6) and P. cervi specimens (n = 8) was amplified and then sequenced. Phylogenetic relationship of the concatenated amino acid sequence data for 12 protein-coding genes of the two rumen flukes and selected members of Trematoda was evaluated using Bayesian inference (BI).
resultsThe complete mt genome of P. leydeni was 14,050 bp in size. Significant nucleotide difference between the P. leydeni mt genome and that of P. cervi (14.7%) was observed. For genetic divergence in ITS-2, sequence difference between P. leydeni and P. cervi was 3.1%, while no sequence variation was detected within each of them. Phylogenetic analysis indicated that P. leydeni and P. cervi are closely-related but distinct rumen flukes.
conclusionsResults of the present study support the proposal that P. leydeni and P. cervi represent two distinct valid species. The mt genome sequences of P. leydeni provide plentiful resources of mitochondrial markers, which can be combined with nuclear markers, for further comparative studies of the biology of P. leydeni and its congeners from China and other countries.
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