ArticleVirology journal2015
Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B.
Article in Virology journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 67 citations in OpenAlex.
- Research note: Molecular epidemiology of Marek's disease virus in China during 2022 and 2023.Poultry science · 2026Article
- A novel recombinant ALV-K carrying ALV-J-derived LTR, pol, and 3'UTR from Chinese indigenous chicken breeds exhibits enhanced replication and increased hemangioma-associated pathogenicity.BMC veterinary research · 2026Article
- Illuminating the pathological, immunohistochemical, and molecular investigations of myelocytomatosis in chicken embryos.Infectious agents and cancer · 2026Article
- Detection of Avian Leukosis Virus Subgroup J (ALV-J) Using RAA and CRISPR-Cas13a Combined with Fluorescence and Lateral Flow Assay.International journal of molecular sciences · 2024Article
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- Immunopathological investigation and genetic evolution of Avian leukosis virus Subgroup-J associated with myelocytomatosis in broiler flocks in Egypt.Virology journal · 2024Article
- Article
- RPA-CRISPR/Cas12a mediated isothermal amplification for visual detection ofFrontiers in cellular and infection microbiology · 2023Article
- Chicken telomerase reverse transcriptase promotes the tumorigenicity of avian leukosis virus subgroup J by regulating the Wnt/β-catenin signaling pathway.Veterinary research · 2022Article
- Development and application of a reverse-transcription recombinase-aided amplification assay for subgroup J Avian leukosis virus.Poultry science · 2022Article
- Enhanced pathogenicity by up-regulation of A20 after avian leukemia subgroup a virus infection.Frontiers in veterinary science · 2022Article
- New rapid detection by using a constant temperature method for avian leukosis viruses.Frontiers in microbiology · 2022Article
- Mutual regulation between chicken telomerase reverse transcriptase and the Wnt/β-catenin signalling pathway inhibits apoptosis and promotes the replication of ALV-J in LMH cells.Veterinary research · 2021Article
- Rapid detection of avian leukosis virus subgroup J by cross-priming amplification.Scientific reports · 2021Article
- Article
- Avian leukosis virus subgroup J and reticuloendotheliosis virus coinfection induced TRIM62 regulation of the actin cytoskeleton.Journal of veterinary science · 2020Article
- Co-infection with avian hepatitis E virus and avian leukosis virus subgroup J as the cause of an outbreak of hepatitis and liver hemorrhagic syndromes in a brown layer chicken flock in China.Poultry science · 2020Article
- Identification of the cDNA Encoding the Growth Hormone Receptor (Frontiers in genetics · 2020Article
- TRIM62 From Chicken as a Negative Regulator of Reticuloendotheliosis Virus Replication.Frontiers in veterinary science · 2020Article
- A cell line resistant to avian leukosis virus subgroup B infection.Poultry science · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSubgroup A, B, and J ALVs are the most prevalent avian leukosis virus (ALV). Our study attempted to develop two SYBR Green I-based real-time PCR (RT-PCR) assays for specific detection of ALV subgroup J (ALV-J) and multiplex detection of ALV subgroups A and B (ALV-A/B), respectively.
resultsThe two assays showed high specificity for ALV-J and ALV-A/B and the sensitivity of the two assays was at least 100 times higher than that of the routine PCR assay. The minimum virus detection limit of virus culture, routine PCR and real-time PCR for detection of ALV-A strain was 10(3) TCID50 units, 10(2) TCID50 units and fewer than 10 TCID50 units, respectively. In addition, the coefficients of variation for intra- and inter-assay were both less than 5%. Forty clinical plasma samples were evaluated by real-time PCR, routine PCR, and virus culture with positive rates of 80% (32/40), 72.5% (29/40) and 62.5% (25/40), respectively. When the assay for detection of ALV-J was used to quantify the viral load of various organ tissues in chicken inoculated by ALV-J strains CHN06 and NX0101, the results exhibited that ALV-J genes could be detected in all organ tissues examined and the highest copies of ALV-J were mainly in heart and kidney samples at 30 weeks post-infection. Except in lung, the virus copies of CHN06 group were higher than that of NX0101 group in various organ tissues.
conclusionsThe SYBR Green I-based real-time RT-PCR assay provides a powerful tool for the detection of ALV and study of virus replication and infection.
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