ArticleRadiation oncology (London, England)2015
Distinct increased outliers among 136 rectal cancer patients assessed by γH2AX.
Article in Radiation oncology (London, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- Relationship between radiosensitivity of peripheral blood lymphocytes, breast cancer cells, and breast cancer stem cells in patients with breast cancer.Biochemistry and biophysics reports · 2026Article
- Influence of Gender on Radiosensitivity during Radiochemotherapy of Advanced Rectal Cancer.Cancers · 2021Article
- Development of a high-throughput γ-H2AX assay based on imaging flow cytometry.Radiation oncology (London, England) · 2019Article
- A novel automatic quantification method for high-content screening analysis of DNA double strand-break response.Scientific reports · 2017Article
- A prospective study on histone γ-H2AX and 53BP1 foci expression in rectal carcinoma patients: correlation with radiation therapy-induced outcome.BMC cancer · 2015Article
- DNA damage in blood lymphocytes in patients after (177)Lu peptide receptor radionuclide therapy.European journal of nuclear medicine and molecular imaging · 2015Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn recent years attention has focused on γH2AX as a very sensitive double strand break indicator. It has been suggested that γH2AX might be able to predict individual radiosensitivity. Our aim was to study the induction and repair of DNA double strand breaks labelled by γH2AX in a large cohort.
methodsIn a prospective study lymphocytes of 136 rectal cancer (RC) patients and 59 healthy individuals were ex vivo irradiated (IR) and initial DNA damage was compared to remaining DNA damage after 2 Gy and 24 hours repair time and preexisting DNA damage in unirradiated lymphocytes. Lymphocytes were immunostained with anti-γH2AX antibodies and microscopic images with an extended depth of field were acquired. γH2AX foci counting was performed using a semi-automatic image analysis software.
resultsDistinct increased values of preexisting and remaining γH2AX foci in the group of RC patients were found compared to the healthy individuals. Additionally there are clear differences within the groups and there are outliers in about 12% of the RC patients after ex vivo IR.
conclusionsThe γH2AX assay has the capability to identify a group of outliers which are most probably patients with increased radiosensitivity having the highest risk of suffering radiotherapy-related late sequelae.
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