ArticleMicrobial cell factories2015
Increased production of inosine and guanosine by means of metabolic engineering of the purine pathway in Ashbya gossypii.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Taste characterization of okara and okara-derived substrates fermented byFood chemistry: X · 2026Article
- Scarless Genetic Engineering ofJournal of microbiology and biotechnology · 2025Article
- 5,6-Dihydro-5,6-Epoxymultiplolide A, Cytosporone C, and Uridine Production byMicroorganisms · 2025Article
- The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine.Microbial cell factories · 2024Article
- Article
- Intermittent Fasting Alleviates Risk Markers in a Murine Model of Ulcerative Colitis by Modulating the Gut Microbiome and Metabolome.Nutrients · 2022Article
- The gateway to guanine nucleotides: Allosteric regulation of IMP dehydrogenases.Protein science : a publication of the Protein Society · 2022Review
- Genomic and Metabolomic Analyses of the Marine FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Genome and Metabolome MS-Based Mining of a Marine Strain ofJournal of fungi (Basel, Switzerland) · 2021Article
- Cross-Talk between Probiotic Nissle 1917 and Human Colonic Epithelium Affects the Metabolite Composition and Demonstrates Host Antibacterial Effect.Metabolites · 2021Article
- New Promoters for Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2021Article
- Metabolomics reveals synergy between Ag and g-CMetabolomics : Official journal of the Metabolomic Society · 2021Article
- Article
- One-vector CRISPR/Cas9 genome engineering of the industrial fungus Ashbya gossypii.Microbial biotechnology · 2019Article
- Metabolic engineering of Ashbya gossypii for deciphering the de novo biosynthesis of γ-lactones.Microbial cell factories · 2019Article
- New biotechnological applications for Ashbya gossypii: Challenges and perspectives.Bioengineered · 2017Review
- Bioproduction of riboflavin: a bright yellow history.Journal of industrial microbiology & biotechnology · 2017Review
- Engineering Ashbya gossypii strains for de novo lipid production using industrial by-products.Microbial biotechnology · 2017Article
- Guanine nucleotide binding to the Bateman domain mediates the allosteric inhibition of eukaryotic IMP dehydrogenases.Nature communications · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInosine and guanosine monophosphate nucleotides are convenient sources of the umami flavor, with attributed beneficial health effects that have renewed commercial interest in nucleotide fermentations. Accordingly, several bacterial strains that excrete high levels of inosine and guanosine nucleosides are currently used in the food industry for this purpose.
resultsIn the present study, we show that the filamentous fungus Ashbya gossypii, a natural riboflavin overproducer, excretes high amounts of inosine and guanosine nucleosides to the culture medium. Following a rational metabolic engineering approach of the de novo purine nucleotide biosynthetic pathway, we increased the excreted levels of inosine up to 27-fold.
conclusionsWe generated Ashbya gossypii strains with improved production titers of inosine and guanosine. Our results point to Ashbya gossypii as the first eukaryotic microorganism representing a promising candidate, susceptible to further manipulation, for industrial nucleoside fermentation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.