ArticleCell communication and signaling : CCS2015
Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers.
Article in Cell communication and signaling : CCS, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- Articular cartilage loss is an unmitigated risk of human spaceflight.NPJ microgravity · 2024Review
- Omics Studies of Specialized Cells and Stem Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Short-term response of primary human meniscus cells to simulated microgravity.Cell communication and signaling : CCS · 2024Article
- Structural and Molecular Changes of Human Chondrocytes Exposed to the Rotating Wall Vessel Bioreactor.Biomolecules · 2023Article
- Transcriptomic response of bioengineered human cartilage to parabolic flight microgravity is sex-dependent.NPJ microgravity · 2023Article
- In Prostate Cancer Cells Cytokines Are Early Responders to Gravitational Changes Occurring in Parabolic Flights.International journal of molecular sciences · 2022Article
- Joint Cartilage in Long-Duration Spaceflight.Biomedicines · 2022Review
- Modifications of Plasma Membrane Organization in Cancer Cells for Targeted Therapy.Molecules (Basel, Switzerland) · 2021Review
- Exposure to hypergravity during zebrafish development alters cartilage material properties and strain distribution.Bone & joint research · 2021Article
- Testing Lab-on-a-Chip Technology for Culturing Human Melanoma Cells under Simulated Microgravity.Cancers · 2021Article
- Influence of Microgravity on Apoptosis in Cells, Tissues, and Other Systems In Vivo and In Vitro.International journal of molecular sciences · 2020Review
- Alteration of Cytoskeleton Morphology and Gene Expression in Human Breast Cancer Cells under Simulated Microgravity.Cell journal · 2020Article
- The Role of C-X-C Chemokine Receptor Type 4 (CXCR4) in Cell Adherence and Spheroid Formation of Human Ewing's Sarcoma Cells under Simulated Microgravity.International journal of molecular sciences · 2019Article
- Changes of Gene Expression in Euglena gracilis Obtained During the 29Scientific reports · 2019Article
- Biomechanical Factors in Psoriatic Disease: Defective Repair Exertion as a Potential Cause. Hypothesis Presentation and Literature Review.ACR open rheumatology · 2019Review
- Thyroid cancer cells in space during the TEXUS-53 sounding rocket mission - The THYROID Project.Scientific reports · 2018Article
- IL-6 and the dysregulation of immune, bone, muscle, and metabolic homeostasis during spaceflight.NPJ microgravity · 2018Review
- Article
- Cartilage breakdown in microgravity-a problem for long-term spaceflight?NPJ Regenerative medicine · 2017Article
- Acute transcriptional up-regulation specific to osteoblasts/osteoclasts in medaka fish immediately after exposure to microgravity.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChondrocytes are the main cellular component of articular cartilage. In healthy tissue, they are embedded in a strong but elastic extracelluar matrix providing resistance against mechanical forces and friction for the joints. Osteoarthritic cartilage, however, disrupted by heavy strain, has only very limited potential to heal. One future possibility to replace damaged cartilage might be the scaffold-free growth of chondrocytes in microgravity to form 3D aggregates.
resultsTo prepare for this, we have conducted experiments during the 20th DLR parabolic flight campaign, where we fixed the cells after the first (1P) and the 31st parabola (31P). Furthermore, we subjected chondrocytes to isolated vibration and hypergravity conditions. Microarray and quantitative real time PCR analyses revealed that hypergravity regulated genes connected to cartilage integrity (BMP4, MMP3, MMP10, EDN1, WNT5A, BIRC3). Vibration was clearly detrimental to cartilage (upregulated inflammatory IL6 and IL8, downregulated growth factors EGF, VEGF, FGF17). The viability of the cells was not affected by the parabolic flight, but showed a significantly increased expression of anti-apoptotic genes after 31 parabolas. The IL-6 release of chondrocytes cultured under conditions of vibration was not changed, but hypergravity (1.8 g) induced a clear elevation of IL-6 protein in the supernatant compared with corresponding control samples.
conclusionTaken together, this study provided new insights into the growth behavior of chondrocytes under short-term microgravity.
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