Evidence map›Paper›PMID 25889661›Full record

ArticleStem cell research & therapy2015

Mesenchymal stem cell-derived microparticles ameliorate peritubular capillary rarefaction via inhibition of endothelial-mesenchymal transition and decrease tubulointerstitial fibrosis in unilateral ureteral obstruction.

Hoon Young Choi, Hyun Gyu Lee, Beom Seok Kim, Sun Hee Ahn, Ara Jung, Mirae Lee, Jung Eun Lee, Hyung Jong Kim, Sung Kyu Ha, Hyeong Cheon Park

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed, 1 pooled it
4.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 1 synthesis or guideline pooled it, 80 citations in OpenAlex.

  1. Pooled it
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  10. Mesenchymal Stem Cells and Their Exocytotic Vesicles.International journal of molecular sciences · 2023
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  14. The role of extracellular vesicles in podocyte autophagy in kidney disease.Journal of cell communication and signaling · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Hoon Young ChoiDepartment of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. hychoi.dr@gmail.com.
Hyun Gyu LeeDepartment of Microbiology and Immunology, Yonsei University College of Medicine, Seoul, Korea. HLEE23@yuhs.ac.
Beom Seok KimDepartment of Internal Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. docbsk@yuhs.ac.
Sun Hee AhnDepartment of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. dkwlxm@hanmail.net.
Ara JungDepartment of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. eeyorelove@hanmail.net.
Mirae LeeDepartment of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. alfo0103@naver.com.
Jung Eun LeeDepartment of Internal Medicine, Yong-In Severance Hospital, Gyeongi-do, Korea. SW0615@yuhs.ac.
Hyung Jong KimDepartment of Internal Medicine, CHA Bundang Medical Center, CHA University, Gyeongi-do, Korea. khj04@cha.ac.kr.
Sung Kyu HaDepartment of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. hask1951@yuhs.ac.
Hyeong Cheon ParkDepartment of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. amp97@yuhs.ac.
Yonsei University · KRSeverance Hospital · KRGangnam Severance Hospital · KRCHA University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionMicroparticles (MPs) derived from kidney-derived mesenchymal stem cells (KMSCs) have recently been reported to ameliorate rarefaction of peritubular capillaries (PTC) in ischemic kidneys via delivery of proangiogenic effectors. This study aimed to investigate whether KMSC-derived MPs show anti-fibrotic effects by ameliorating endothelial-to-mesenchymal transition (EndoMT) in human umbilical vein endothelial cells (HUVEC) in vitro and by preserving PTC in kidneys with unilateral ureteral obstruction (UUO) in vivo.

methodsMPs isolated from the supernatants of KMSC were co-cultured with HUVEC to assess their in vitro biologic effects on endothelial cells. Mice were treated with MPs via the tail vein after UUO injury to assess their anti-fibrotic and PTC sparing effects. Renal tubulointerstitial damage and inflammatory cell infiltration were examined with Masson's trichrome, F4/80 and α-smooth muscle actin (α-SMA) staining and PTC rarefaction index was determined by CD31 staining.

resultsKMSC-derived MPs significantly ameliorated EndoMT and improved in vitro proliferation of TGF-β1 treated HUVEC. In vivo administration of KMSC-derived MPs significantly inhibited EndoMT of PTC endothelial cells and improved PTC rarefaction in UUO kidneys. Furthermore, administration of KMSC-derived MPs inhibited inflammatory cell infiltration as well as tubulointerstitial fibrosis in UUO mice as demonstrated by decreased F4/80 and α-SMA-positive cells and Masson's trichrome staining, respectively.

conclusionsOur results suggest that KMSC-derived MPs ameliorate PTC rarefaction via inhibition of EndoMT and protect against progression of renal damage by inhibiting tubulointerstitial fibrosis.

Indexed as

ActinsAnimalsAntigens, DifferentiationCell-Derived MicroparticlesCell LineCell ProliferationCoculture TechniquesDisease Models, AnimalEpithelial-Mesenchymal TransitionFibrosisHumansHuman Umbilical Vein Endothelial CellsInflammationKidney TubulesMesenchymal Stem CellsMiceActinsalpha-smooth muscle actin, mouseAntigens, Differentiationmonocyte-macrophage differentiation antigenTransforming Growth Factor beta

Identifiers

PMID25889661
PMCPMC4393614
OpenAlexW2135682943

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.