Evidence map›Paper›PMID 25889567›Full record

ArticleParasites & vectors2015

Genetic structuring and fixed polymorphisms in the gene period among natural populations of Lutzomyia longipalpis in Brazil.

César Raimundo Lima Costa, Moises Thiago de Souza Freitas, Carlos Alberto Santiago Figueirêdo, Nádia Consuelo Aragão, Lidiane Gomes da Silva, Carlos Brisola Marcondes, Raimundo Vieira Dias, Tereza Cristina Leal-Balbino, Manuela Barbosa Rodrigues Souza, Marcelo Ramalho-Ortigão and 1 more

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Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

César Raimundo Lima CostaDepartamento de Genética, Universidade Federal de Pernambuco, Avenida da Engenharia S/N, Cidade Universitária, 50.740-600, Recife, Pernambuco, Brazil. cjrs@ig.com.br.
Moises Thiago de Souza FreitasDepartamento de Genética, Universidade Federal de Pernambuco, Avenida da Engenharia S/N, Cidade Universitária, 50.740-600, Recife, Pernambuco, Brazil. moisestsf@hotmail.com.
Carlos Alberto Santiago FigueirêdoDepartamento de Genética, Universidade Federal de Pernambuco, Avenida da Engenharia S/N, Cidade Universitária, 50.740-600, Recife, Pernambuco, Brazil. cjsantifigo@hotmail.com.
Nádia Consuelo AragãoDepartamento de Genética, Universidade Federal de Pernambuco, Avenida da Engenharia S/N, Cidade Universitária, 50.740-600, Recife, Pernambuco, Brazil. nadibr2@yahoo.com.br.
Lidiane Gomes da SilvaDepartamento de Genética, Universidade Federal de Pernambuco, Avenida da Engenharia S/N, Cidade Universitária, 50.740-600, Recife, Pernambuco, Brazil. lidianegomesp@hotmail.com.
Carlos Brisola MarcondesDepartamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Campus Reitor João David Ferreira Lima, 88040-900, Florianópolis, Santa Catarina, Brazil. cbrisolamarcondes@gmail.com.
Raimundo Vieira DiasCentro de Controle de Zoonoses, Rua Finlândia S/N, Parque Silvana II, 62010-970, Sobral, Ceara, Brazil. netovet1@hotmail.com.
Tereza Cristina Leal-BalbinoDepartamento de Microbiologia, Centro de Pesquisas Aggeu Magalhaes, Avenida Professor Moraes Rego S/N, Cidade Universitária, 50740-465, Recife, Pernambuco, Brasil. cristina@cpqam.fiocruz.br.
Manuela Barbosa Rodrigues SouzaDepartamento de Genética, Universidade Federal de Pernambuco, Avenida da Engenharia S/N, Cidade Universitária, 50.740-600, Recife, Pernambuco, Brazil. manubrsouza@gmail.com.
Marcelo Ramalho-OrtigãoDepartment of Entomology, Kansas State University, W. Waters Hall 123, 66506-400, Manhattan, KS, USA. mortigao@k-state.edu.
Valdir de Queiroz BalbinoDepartamento de Genética, Universidade Federal de Pernambuco, Avenida da Engenharia S/N, Cidade Universitária, 50.740-600, Recife, Pernambuco, Brazil. vqbalbino@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEven one hundred years after being originally identified, aspects of the taxonomy of the sand fly Lutzomyia longipalpis, the principal vector of Leishmania infantum in the Americas, remain unresolved for Brazilian populations of this vector. The diversity of morphological, behavioral, biochemical, and ethological characters, as well as the genetic variability detected by molecular markers are indicative of the presence of a complex of species.

methodsIn this study, a 525 bp fragment of the period gene was used to evaluate sympatric populations of L. longipalpis. A combination of probabilistic methods such as maximum likelihood and genetic assignment approach to investigate sympatric species of L. longipalpis were applied in three populations of Northeast Brazil.

resultsFixed polymorphisms in geographically isolated populations of L. longipalpis from two localities in the state of Ceará and one in the state of Pernambuco, Brazil, was identified in a 525 bp fragment of the gene period (per). Our results suggest a direct relationship between the number of spots found in males' tergites and the genetic variation in cryptic species of L. longipalpis. The fragment used in this study revealed the nature of the ancestral morphotype 1S.

conclusionNew polymorphisms were identified in the gene per which can be used as a genetic barcode to sympatric taxonomy of L. longipalpis. The per gene fragment confirmed the presence of two siblings species of L. longipalpis in Sobral and showed that these same species are present in two other localities, representing an expansion within the L. longipalpis species complex with regards to the states of Ceará and Pernambuco.

Indexed as

Animal DistributionAnimalsBase SequenceBrazilDNAGene Expression RegulationInsect ProteinsMolecular Sequence DataPhylogenyPolymorphism, GeneticPsychodidaeDNAInsect Proteins

Identifiers

PMID25889567
PMCPMC4409732

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.