ArticleStem cell research & therapy2015
Proteomic analysis of porcine mesenchymal stem cells derived from bone marrow and umbilical cord: implication of the proteins involved in the higher migration capability of bone marrow mesenchymal stem cells.
Article in Stem cell research & therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Advances in porcine stem cell research and their applications in agriculture.Frontiers in cell and developmental biology · 2026Review
- Article
- Validation of a color deconvolution method to quantify MSC tri-lineage differentiation across species.Frontiers in veterinary science · 2022Article
- Integrated analysis of DNA methylome and transcriptome reveals the differences in biological characteristics of porcine mesenchymal stem cells.BMC genomic data · 2021Article
- Evaluation of Allogeneic Bone-Marrow-Derived and Umbilical Cord Blood-Derived Mesenchymal Stem Cells to Prevent the Development of Osteoarthritis in An Equine Model.International journal of molecular sciences · 2021Article
- Advances in translational orthopaedic research with species-specific multipotent mesenchymal stromal cells derived from the umbilical cord.Histology and histopathology · 2021Review
- MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From Osteoarthritis.Frontiers in cell and developmental biology · 2021Article
- Neovascularization: The Main Mechanism of MSCs in Ischemic Heart Disease Therapy.Frontiers in cardiovascular medicine · 2021Review
- Three-dimensional environment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitors.Stem cells translational medicine · 2020Article
- Proteomic analysis of the response of porcine adrenal gland to heat stress.Research in veterinary science · 2019Article
- Nanobiosensing Platforms for Real-Time and Non-Invasive Monitoring of Stem Cell Pluripotency and Differentiation.Sensors (Basel, Switzerland) · 2018Review
- Bibliometric analysis of global scientific activity on umbilical cord mesenchymal stem cells: a swiftly expanding and shifting focus.Stem cell research & therapy · 2018Review
- Cell viability assessed in a reproducible model of human osteoblasts derived from human adipose-derived stem cells.PloS one · 2018Article
- Wharton's Jelly Mesenchymal Stromal Cells Support the Expansion of Cord Blood-derived CD34Cell transplantation · 2018Article
- Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry.Scientific reports · 2017Article
- Mesenchymal stromal cells' role in tumor microenvironment: involvement of signaling pathways.Cancer biology & medicine · 2017Article
- iTRAQ-based proteomic analysis reveals key proteins affecting muscle growth and lipid deposition in pigs.Scientific reports · 2017Article
- Mouse Mesenchymal Progenitor Cells Expressing Adipogenic and Osteogenic Transcription Factors Suppress the Macrophage Inflammatory Response.Stem cells international · 2017Article
- Stromal cell-derived factor-1α and transforming growth factor-βAmerican journal of translational research · 2017Article
- Uncovering stem cell differentiation factors for salivary gland regeneration by quantitative analysis of differential proteomes.PloS one · 2017Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
introductionMesenchymal stem cells (MSCs) have the ability to proliferate in vivo with a large variety of differentiation potentials and therefore are widely used as an ideal material for cell therapy. MSCs derived from pig and human sources are similar in many aspects, such as cell immunophenotype and functional characteristics. However, differences in proteomics and the molecular mechanisms of cell functions between porcine bone marrow MSCs (BM-MSCs) and umbilical cord MSCs (UC-MSCs) are largely unknown. To the best of our knowledge, MSCs collected from different tissue have specific phenotype and differentiation ability in response to microenvironment, known as a niche.
methodsPorcine BM-MSCs and UC-MSCs were evaluated with flow cytometric and adipogenic and osteogenic differentiation analyses. We used isobaric tagging for relative and absolute quantitation (iTRAQ), combined with liquid chromatography-tandem mass spectrometry, to identify differentially expressed proteins (DEPs) between these two types of MSCs. Kyoto Encyclopedia of Genes and Genomes pathway and phenotype analyses were used to understand the links between cell migration ability and DEPs.
resultsTwo separate iTRAQ experiments were conducted, identifying 95 DEPs (95% confidence interval). Five of these proteins were verified by Western blotting. These 95 DEPs were classified in terms of biological regulation, metabolic process, developmental process, immune system process, reproduction, death, growth, signaling, localization, response to stimulus, biological adhesion, and cellular component organization. Our study is the first to show results indicating that porcine BM-MSCs have a higher migration capability than UC-MSCs. Finally, one of the DEPs, Vimentin, was verified to have a positive role in MSC migration.
conclusionsThese results represent the first attempt to use proteomics specifically targeted to porcine MSCs of different tissues. The identified components should help reveal a variety of tissue-specific functions in tissue-derived MSC populations and could serve as important tools for the regeneration of particular tissues in future stem cell-based tissue engineering studies using animal models.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.